| Autor: |
Veerasubramanian PK; Department of Biomedical Engineering, University of California-Irvine, Irvine, CA 92697, USA.; UCI Edwards Lifesciences Foundation Cardiovascular Innovation and Research Center (CIRC), University of California-Irvine, Irvine, CA 92697, USA., Joe VC; Department of Surgery, University of California-Irvine, Irvine, CA 92697, USA., Liu WF; Department of Biomedical Engineering, University of California-Irvine, Irvine, CA 92697, USA.; UCI Edwards Lifesciences Foundation Cardiovascular Innovation and Research Center (CIRC), University of California-Irvine, Irvine, CA 92697, USA.; Department of Chemical and Biomolecular Engineering, University of California-Irvine, Irvine, CA 92697, USA.; Institute for Immunology, University of California-Irvine, Irvine, CA 92697, USA.; Department of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, CA 92697, USA., Downing TL; Department of Biomedical Engineering, University of California-Irvine, Irvine, CA 92697, USA.; UCI Edwards Lifesciences Foundation Cardiovascular Innovation and Research Center (CIRC), University of California-Irvine, Irvine, CA 92697, USA.; NSF-Simons Center for Multiscale Cell Fate Research, University of California-Irvine, Irvine, CA 92697, USA.; Department of Microbiology and Molecular Genetics, University of California-Irvine, Irvine, CA 92697, USA. |
| Abstrakt: |
Macrophages are innate immune cells that help wounds heal. Here, we study the potential immunomodulatory effects of negative-pressure wound therapy (NPWT) materials on the macrophage inflammatory response. We compared the effects of two materials, Granufoam™ (GF) and Veraflo Cleanse™ (VC), on macrophage function in vitro. We find that both materials cause reduced expression of inflammatory genes, such as TNF and IL1B, in human macrophages stimulated with bacterial lipopolysaccharide (LPS) and interferon-gamma (IFNγ). Relative to adherent glass control surfaces, VC discourages macrophage adhesion and spreading, and may potentially sequester LPS/IFNγ and cytokines that the cells produce. GF, on the other hand, was less suppressive of inflammation, supported macrophage adhesion and spreading better than VC, and sequestered lesser quantities of LPS/IFNγ in comparison to VC. The control dressing material cotton gauze (CT) was also immunosuppressive, capable of TNF-α retention and LPS/IFNγ sequestration. Our findings suggest that NPWT material interactions with cells, as well as soluble factors including cytokines and LPS, can modulate the immune response, independent of vacuum application. We have also established methodological strategies for studying NPWT materials and reveal the potential utility of cell-based in vitro studies for elucidating biological effects of NPWT materials. |
