Combination of High-Resolution Multistage Ion Mobility and Tandem MS with High Energy of Activation to Resolve the Structure of Complex Chemoenzymatically Synthesized Glycans.

Autor: Ropartz D; INRAE, UR BIA, F-44316 Nantes, France.; INRAE, BIBS Facility, F-44316 Nantes, France., Fanuel M; INRAE, UR BIA, F-44316 Nantes, France.; INRAE, BIBS Facility, F-44316 Nantes, France., Ollivier S; INRAE, UR BIA, F-44316 Nantes, France.; INRAE, BIBS Facility, F-44316 Nantes, France., Lissarrague A; INRAE, UR BIA, F-44316 Nantes, France.; INRAE, BIBS Facility, F-44316 Nantes, France., Benkoulouche M; Toulouse Biotechnology Institute, TBI, Université de Toulouse, CNRS, INRAE, INSA, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 04, France., Mulard LA; Institut Pasteur, Université de Paris, CNRS UMR3523, Unité de Chimie des Biomolécules, 28 rue du Dr Roux, 75724 Paris Cedex 15, France., André I; Toulouse Biotechnology Institute, TBI, Université de Toulouse, CNRS, INRAE, INSA, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 04, France., Guieysse D; Toulouse Biotechnology Institute, TBI, Université de Toulouse, CNRS, INRAE, INSA, 135 Avenue de Rangueil, F-31077 Toulouse Cedex 04, France., Rogniaux H; INRAE, UR BIA, F-44316 Nantes, France.; INRAE, BIBS Facility, F-44316 Nantes, France.
Jazyk: angličtina
Zdroj: Analytical chemistry [Anal Chem] 2022 Feb 01; Vol. 94 (4), pp. 2279-2287. Date of Electronic Publication: 2022 Jan 20.
DOI: 10.1021/acs.analchem.1c04982
Abstrakt: Carbohydrates, in particular microbial glycans, are highly structurally diverse biomolecules, the recognition of which governs numerous biological processes. Of special interest, glycans of known monosaccharide composition feature multiple possible isomers, differentiated by the anomerism and position of their glycosidic linkages. Robust analytical tools able to circumvent this extreme structural complexity are increasing in demand to ensure not only the correct determination of naturally occurring glycans but also to support the rapid development of enzymatic and chemoenzymatic glycan synthesis. In support to the later, we report the use of complementary strategies based on mass spectrometry (MS) to evaluate the ability of 14 engineered mutants of sucrose-utilizing α-transglucosylases to produce type/group-specific Shigella flexneri pentasaccharide bricks from a single lightly protected non-natural tetrasaccharide acceptor substrate. A first analysis of the reaction media by UHPLC coupled to high-accuracy MS led to detect six reaction products of enzymatic glucosylation out of the eight possible ones. A seventh structure was evidenced by an additional step of ion mobility at a resolving power ( R p ) of approximately 100. Finally, a R p of about 250 in ion mobility made it possible to detect the eighth and last of the expected structures. Complementary to these measurements, tandem MS with high activation energy charge transfer dissociation (CTD) allowed us to unambiguously characterize seven regioisomers out of the eight possible products of enzymatic glucosylation. This work illustrates the potential of the recently described powerful IMS and CTD-MS methods for the precise structural characterization of complex glycans.
Databáze: MEDLINE
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