Persistence of Anti-SARS-CoV-2 Antibodies in Long Term Care Residents Over Seven Months After Two COVID-19 Outbreaks.

Autor:	Tanunliong G; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada., Liu A; Department of Experimental Medicine, University of British Columbia, Vancouver, BC, Canada., Vijh R; Office of the Chief Medical Health Officer, Vancouver Coastal Health, Vancouver, BC, Canada.; School of Population and Public Health, University of British Columbia, Vancouver, BC, Canada., Pidduck T; British Columbia Centre for Disease Control (BCCDC) Public Health Laboratory, Vancouver, BC, Canada., Kustra J; British Columbia Centre for Disease Control (BCCDC) Public Health Laboratory, Vancouver, BC, Canada., Márquez AC; British Columbia Centre for Disease Control (BCCDC) Public Health Laboratory, Vancouver, BC, Canada., Choi A; Office of the Chief Medical Health Officer, Vancouver Coastal Health, Vancouver, BC, Canada., McLennan M; British Columbia Centre for Disease Control (BCCDC) Public Health Laboratory, Vancouver, BC, Canada., Hayden A; Office of the Chief Medical Health Officer, Vancouver Coastal Health, Vancouver, BC, Canada., Kearney C; Haro Park Centre, Vancouver, BC, Canada., Gantt S; Centre de Recherche de Centre Hospitalier Universitaire (CHU) Sainte-Justine, Département de microbiologie, infectiologie et immunologie, Université de Montréal, Montréal, QC, Canada., Krajden M; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.; British Columbia Centre for Disease Control (BCCDC) Public Health Laboratory, Vancouver, BC, Canada., Morshed M; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.; British Columbia Centre for Disease Control (BCCDC) Public Health Laboratory, Vancouver, BC, Canada., Jassem AN; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.; British Columbia Centre for Disease Control (BCCDC) Public Health Laboratory, Vancouver, BC, Canada., Sekirov I; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.; British Columbia Centre for Disease Control (BCCDC) Public Health Laboratory, Vancouver, BC, Canada.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2022 Jan 03; Vol. 12, pp. 775420. Date of Electronic Publication: 2022 Jan 03 (Print Publication: 2021).
DOI:	10.3389/fimmu.2021.775420
Abstrakt:	Background: As part of the public health outbreak investigations, serological surveys were carried out following two COVID-19 outbreaks in April 2020 and October 2020 in one long term care facility (LTCF) in British Columbia, Canada. This study describes the serostatus of the LTCF residents and monitors changes in their humoral response to SARS-CoV-2 and other human coronaviruses (HCoV) over seven months. Methods: A total of 132 serum samples were collected from all 106 consenting residents (aged 54-102) post-first outbreak (N=87) and post-second outbreak (N=45) in one LTCF; 26/106 participants provided their serum following both COVID-19 outbreaks, permitting longitudinal comparisons between surveys. Health-Canada approved commercial serologic tests and a pan-coronavirus multiplexed immunoassay were used to evaluate antibody levels against the spike protein, nucleocapsid, and receptor binding domain (RBD) of SARS-CoV-2, as well as the spike proteins of HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43. Statistical analyses were performed to describe the humoral response to SARS-CoV-2 among residents longitudinally. Findings: Survey findings demonstrated that among the 26 individuals that participated in both surveys, all 10 individuals seropositive after the first outbreak continued to be seropositive following the second outbreak, with no reinfections identified among them. SARS-CoV-2 attack rate in the second outbreak was lower (28.6%) than in the first outbreak (40.2%), though not statistically significant (P>0.05). Gradual waning of anti-nucleocapsid antibodies to SARS-CoV-2 was observed on commercial (median Δ=-3.7, P=0.0098) and multiplexed immunoassay (median Δ=-169579, P=0.014) platforms; however, anti-spike and anti-receptor binding domain (RBD) antibodies did not exhibit a statistically significant decline over 7 months. Elevated antibody levels for beta-HCoVs OC43 (P<0.0001) and HKU1 (P=0.0027) were observed among individuals seropositive for SARS-CoV-2 compared to seronegative individuals. Conclusion: Our study utilized well-validated serological platforms to demonstrate that humoral responses to SARS-CoV-2 persisted for at least 7 months. Elevated OC43 and HKU1 antibodies among SARS-CoV-2 seropositive individuals may be attributed to cross reaction and/or boosting of humoral response. Competing Interests: SG reports having received research support and/or consulting fees from Moderna, Merck, GSK, VBI Vaccines, and Meridian Biosciences. No products of these companies were used in the study. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Tanunliong, Liu, Vijh, Pidduck, Kustra, Márquez, Choi, McLennan, Hayden, Kearney, Gantt, Krajden, Morshed, Jassem and Sekirov.)
Databáze:	MEDLINE
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