Insights into the genetic diversity of an underutilized Indian legume, Vigna stipulacea (Lam.) Kuntz., using morphological traits and microsatellite markers.

Autor: Gore PG; Division of Plant Genetic Resources, ICAR - Indian Agricultural Research Institute, New Delhi, India.; ICAR-National Bureau of Plant Genetic Resources, New Delhi, India., Gupta V; ICAR-National Bureau of Plant Genetic Resources, New Delhi, India., Singh R; ICAR-National Bureau of Plant Genetic Resources, New Delhi, India., Tripathi K; ICAR-National Bureau of Plant Genetic Resources, New Delhi, India., Kumar R; ICAR-National Bureau of Plant Genetic Resources, New Delhi, India., Kumari G; ICAR-Indian Institute of Pulses Research, Kanpur, Uttar Pradesh, India., Madhavan L; ICAR-National Bureau of Plant Genetic Resources, Thrissur, Kerala, India., Dikshit HK; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India., Venkateswaran K; ICAR-National Bureau of Plant Genetic Resources, Hyderabad, Telangana, India., Pandey A; ICAR-National Bureau of Plant Genetic Resources, New Delhi, India., Singh N; ICAR-National Bureau of Plant Genetic Resources, New Delhi, India., Bhat KV; ICAR-National Bureau of Plant Genetic Resources, New Delhi, India., Nair RM; World Vegetable Center, South and Central Asia, Hyderabad, Telangana, India., Pratap A; ICAR-Indian Institute of Pulses Research, Kanpur, Uttar Pradesh, India.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Jan 19; Vol. 17 (1), pp. e0262634. Date of Electronic Publication: 2022 Jan 19 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0262634
Abstrakt: Vigna stipulacea (Lam.) Kuntz., commonly known as Minni payaru is an underutilized legume species and has a great potential to be utilized as food crop. To evaluate and select the best germplasm to be harnessed in the breeding programme, we assessed the genetic diversity of V. stipulacea (94 accessions) conserved in the Indian National Genebank, based on morphological traits and microsatellite markers. Significant variation was recorded for the morphological traits studied. Euclidean distance using UPGMA method grouped all accessions into two major clusters. Accessions were identified for key agronomic traits such as, early flowering (IC331436, IC251436, IC331437); long peduncle length (IC553518, IC550531, IC553557, IC553540, IC550532, IC553564); and more number of seeds per pod (IC553529, IC622865, IC622867, IC553528). To analyse the genetic diversity among the germplasm 33 SSR primers were used anda total of 116 alleles were detected. The number of alleles varied from two to seven, with an average of 3.52 per loci. The polymorphic information content values varied from 0.20 to 0.74, with a mean of 0.40. The high number of alleles per locus and the allelic diversity in the studied germplasm indicated a relatively wider genetic base of V. stipulacea. Phylogenetic analysis clustered accessions into seven clades. Population structure analysis grouped them into five genetic groups, which were partly supported by PCoA and phylogenetic tree. Besides, PCoA and AMOVA also decoded high genetic diversity among the V. stipulacea accessions. Thus, morphological and microsatellite markers distinguished V. stipulacea accessions and assessed their genetic diversity efficiently. The identified promising accessions can be utilized in Vigna improvement programme through introgression breeding and/or can be used for domestication and enhanced utilization of V. stipulacea.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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