Differential expression of CD11c defines two types of tissue-resident macrophages with different origins in steady-state salivary glands.

Autor: Lu L; Division of Oral Immunology, Department of Ecological Dentistry, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan., Kuroishi T; Division of Oral Immunology, Department of Ecological Dentistry, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan., Tanaka Y; Department of Dental Anesthesiology and Pain Management, Tohoku University Hospital, Sendai, 980-8574, Japan., Furukawa M; International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan., Nochi T; International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan., Sugawara S; Division of Oral Immunology, Department of Ecological Dentistry, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan. shunji.sugawara.d5@tohoku.ac.jp.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jan 18; Vol. 12 (1), pp. 931. Date of Electronic Publication: 2022 Jan 18.
DOI: 10.1038/s41598-022-04941-5
Abstrakt: Gland macrophages are primed for gland development and functions through interactions within their niche. However, the phenotype, ontogeny, and function of steady-state salivary gland (SG) macrophages remain unclear. We herein identified CD11c + and CD11c - subsets among CD64 + macrophages in steady-state murine SGs. CD11c - macrophages were predominant in the SGs of embryonic and newborn mice and decreased with advancing age. CD11c + macrophages were rarely detected in the embryonic period, but rapidly expanded after birth. CD11c + , but not CD11c - , macrophage numbers decreased in mice treated with a CCR2 antagonist, suggesting that CD11c + macrophages accumulate from bone marrow-derived progenitors in a CCR2-dependent manner, whereas CD11c - macrophages were derived from embryonic progenitors in SGs. CD11c + and CD11c - macrophages strongly expressed colony-stimulating factor (CSF)-1 receptor, the injection of an anti-CSF-1 receptor blocking antibody markedly reduced both subsets, and SGs strongly expressed CSF-1, indicating the dependency of SG resident macrophage development on CSF-1. The phagocytic activity of SG macrophages was extremely weak; however, the gene expression profile of SG macrophages indicated that SG macrophages regulate gland development and functions in SGs. These results suggest that SG CD11c + and CD11c - macrophages are developed and instructed to perform SG-specific functions in steady-state SGs.
(© 2022. The Author(s).)
Databáze: MEDLINE
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