Nestin and NG2 transgenes reveal two populations of perivascular cells stimulated by photobiomodulation.

Autor: Gomes NA; Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., do Valle IB; Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Gleber-Netto FO; Department of Head & Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA., Silva TA; Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Oliveira HMC; Department of Morphology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., de Oliveira RF; Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Ferreira LAQ; Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Castilho LS; Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Reis PHRG; Ohlab, Associação Mineira de Reabilitação, Belo Horizonte, Brazil., Prazeres PHDM; Departament of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Menezes GB; Department of Morphology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., de Magalhães CS; Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Mesquita RA; Department of Head & Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA., Marques MM; Postgraduation Program in Dentistry, Ibirapuera University, São Paulo, Brazil., Birbrair A; Departament of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Diniz IMA; Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Jazyk: angličtina
Zdroj: Journal of cellular physiology [J Cell Physiol] 2022 Apr; Vol. 237 (4), pp. 2198-2210. Date of Electronic Publication: 2022 Jan 17.
DOI: 10.1002/jcp.30680
Abstrakt: Pericytes and glial cells are known to collaborate in dental pulp tissue repair. Cell-based therapies that stimulate these stromal components may be of therapeutic relevance for partially vital dental pulp conditions. This study aimed to examine the early effect of photobiomodulation (PBM) in pericytes from experimentally injured pulp tissue. To accomplish this, we used the Nestin-GFP/NG2-DsRed mice, which could allow the identification of distinct pericyte phenotypes. We discovered the presence of two pericytes subsets within the dental pulp, the Nestin + NG2 + (type-2) and Nestin - NG2 + (type-1). Upon injury, PBM treatment led to a significant increase in Nestin + cells and pericytes. This boost was mainly conferred by the more committed pericyte subset (NestinNG2 + ). PBM also stimulated terminal blood vessels sprouting adjacent to the injury site while maintaining signs of pulp vitality. In vitro, PBM induced VEGF upregulation, improved dental pulp cells proliferation and migration, and favored their mineralization potential. Herein, different subsets of perivascular cells were unveiled in the pulp tissue. PBM enhanced not only NG2 + cells but nestin-expressing progenitors in the injured dental pulp.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: