Aging influence on pulmonary and systemic inflammation and neural metabolomics arising from pulmonary multi-walled carbon nanotube exposure in apolipoprotein E-deficient and C57BL/6 female mice.

Autor: Young TL; Department of Pharmaceutical Sciences, University of New Mexico, Albuquerque, NM, USA., Scieszka D; Department of Pharmaceutical Sciences, University of New Mexico, Albuquerque, NM, USA., Begay JG; Department of Pharmaceutical Sciences, University of New Mexico, Albuquerque, NM, USA., Lucas SN; Department of Pharmaceutical Sciences, University of New Mexico, Albuquerque, NM, USA., Herbert G; Department of Pharmaceutical Sciences, University of New Mexico, Albuquerque, NM, USA., Zychowski K; College of Nursing, University of New Mexico, Albuquerque, NM, USA., Hunter R; Department of Pharmaceutical Sciences, University of New Mexico, Albuquerque, NM, USA., Salazar R; Department of Pharmaceutical Sciences, University of New Mexico, Albuquerque, NM, USA., Ottens AK; Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA, USA., Erdely A; Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, Morgantown, WV, USA., Gu H; College of Health Solutions, Arizona State University, Phoenix, AZ, USA.; Center for Translational Science, Florida International University, Port St. Lucie, FL, USA., Campen MJ; Department of Pharmaceutical Sciences, University of New Mexico, Albuquerque, NM, USA.
Jazyk: angličtina
Zdroj: Inhalation toxicology [Inhal Toxicol] 2023 Mar-Apr; Vol. 35 (3-4), pp. 86-100. Date of Electronic Publication: 2022 Jan 17.
DOI: 10.1080/08958378.2022.2026538
Abstrakt: Objective: Environmental exposures exacerbate age-related pathologies, such as cardiovascular and neurodegenerative diseases. Nanoparticulates, and specifically carbon nanomaterials, are a fast-growing contributor to the category of inhalable pollutants, whose risks to health are only now being unraveled. The current study assessed the exacerbating effect of age on multiwalled-carbon nanotube (MWCNT) exposure in young and old C57BL/6 and ApoE -/- mice.
Materials and Methods: Female C57BL/6 and apolipoprotein E-deficient (ApoE -/- ) mice, aged 8 weeks and 15 months, were exposed to 0 or 40 µg MWCNT via oropharyngeal aspiration. Pulmonary inflammation, inflammatory bioactivity of serum, and neurometabolic changes were assessed at 24 h post-exposure.
Results: Pulmonary neutrophil infiltration was induced by MWCNT in bronchoalveolar lavage fluid in both C57BL/6 and ApoE -/- . Macrophage counts decreased with MWCNT exposure in ApoE -/- mice but were unaffected by exposure in C57BL/6 mice. Older mice appeared to have greater MWCNT-induced total protein in lavage fluid. BALF cytokines and chemokines were elevated with MWCNT exposure, but CCL2, CXCL1, and CXCL10 showed reduced responses to MWCNT in older mice. However, no significant serum inflammatory bioactivity was detected. Cerebellar metabolic changes in response to MWCNT were modest, but age and strain significantly influenced metabolite profiles assessed. ApoE -/- mice and older mice exhibited less robust metabolite changes in response to exposure, suggesting a reduced health reserve.
Conclusions: Age influences the pulmonary and neurological responses to short-term MWCNT exposure. However, with only the model of moderate aging (15 months) in this study, the responses appeared modest compared to inhaled toxicant impacts in more advanced aging models.
Databáze: MEDLINE
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