Hyperammonemia-induced changes in the cerebral transcriptome and proteome.
Autor: | Schrimpf A; Clinic for Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University Düsseldorf, Germany., Knappe O; Clinic for Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University Düsseldorf, Germany., Qvartskhava N; Clinic for Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University Düsseldorf, Germany., Poschmann G; Institute for Molecular Medicine, Proteome Research, Medical Faculty and University Hospital Düsseldorf, Heinrich-Heine-University Düsseldorf, Germany., Stühler K; Institute for Molecular Medicine, Proteome Research, Medical Faculty and University Hospital Düsseldorf, Heinrich-Heine-University Düsseldorf, Germany; Molecular Proteomics Laboratory, BMFZ, Heinrich-Heine-University Düsseldorf, Germany., Bidmon HJ; Cécile & Oscar Vogt Institute for Brain Research, Heinrich-Heine-University Düsseldorf, Germany., Luedde T; Clinic for Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University Düsseldorf, Germany., Häussinger D; Clinic for Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University Düsseldorf, Germany. Electronic address: haeussin@uni-duesseldorf.de., Görg B; Clinic for Gastroenterology, Hepatology and Infectious Diseases, Heinrich-Heine-University Düsseldorf, Germany. Electronic address: Boris.Goerg@uni-duesseldorf.de. |
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Jazyk: | angličtina |
Zdroj: | Analytical biochemistry [Anal Biochem] 2022 Mar 15; Vol. 641, pp. 114548. Date of Electronic Publication: 2022 Jan 12. |
DOI: | 10.1016/j.ab.2022.114548 |
Abstrakt: | Molecular alterations underlying cerebral impairment in hyperammonemic disorders such as in hepatic encephalopathy (HE) are only poorly understood. Using transcriptomics and proteomics on brains of mice with systemic hyperammonemia resulting from knockout of hepatic glutamine synthetase (LGS-KO) we identified up to 214 genes and 34 proteins whose expressions were altered in brains of LGS-KO mice in a brain region-specific way. Differentially expressed genes were enriched for those related to oxidative stress, cell proliferation, heme metabolism and others. Due to their particularly high expression changes, coactivator associated arginine methyltransferase 1 (CARM1), TROVE2 and Lipocalin-2 (LCN2) were selected for further analyses. All selected candidates were expressed by astrocytes in rodent brain and challenging cultured astrocytes with NH (Copyright © 2022 Elsevier Inc. All rights reserved.) |
Databáze: | MEDLINE |
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