Investigating Membrane-Mediated Antimicrobial Peptide Interactions with Synchrotron Radiation Far-Infrared Spectroscopy.

Autor: Hornemann A; Department 7.1 Radiometry with Synchrotron Radiation and, Department 7.2 X-Ray Metrology with Synchrotron Radiation, Physikalisch-Technische Bundesanstalt (PTB), Abbestr. 2-12, 10587, Berlin, Germany., Eichert DM; ELETTRA - Sincrotrone Trieste, S.S.14 Km 163.5 in Area Science Park, 34149, Basovizza, Trieste, Italy., Hoehl A; Department 7.1 Radiometry with Synchrotron Radiation and, Department 7.2 X-Ray Metrology with Synchrotron Radiation, Physikalisch-Technische Bundesanstalt (PTB), Abbestr. 2-12, 10587, Berlin, Germany., Tiersch B; Universität Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany., Ulm G; Department 7.1 Radiometry with Synchrotron Radiation and, Department 7.2 X-Ray Metrology with Synchrotron Radiation, Physikalisch-Technische Bundesanstalt (PTB), Abbestr. 2-12, 10587, Berlin, Germany., Ryadnov MG; National Physical Laboratory, Hampton Rd, Teddington, Middlesex, TW11 0LW, UK., Beckhoff B; Department 7.1 Radiometry with Synchrotron Radiation and, Department 7.2 X-Ray Metrology with Synchrotron Radiation, Physikalisch-Technische Bundesanstalt (PTB), Abbestr. 2-12, 10587, Berlin, Germany.
Jazyk: angličtina
Zdroj: Chemphyschem : a European journal of chemical physics and physical chemistry [Chemphyschem] 2022 Feb 16; Vol. 23 (4), pp. e202100815. Date of Electronic Publication: 2022 Jan 14.
DOI: 10.1002/cphc.202100815
Abstrakt: Synchrotron radiation-based Fourier transform infrared spectroscopy enables access to vibrational information from mid over far infrared to even terahertz domains. This information may prove critical for the elucidation of fundamental bio-molecular phenomena including folding-mediated innate host defence mechanisms. Antimicrobial peptides (AMPs) represent one of such phenomena. These are major effector molecules of the innate immune system, which favour attack on microbial membranes. AMPs recognise and bind to the membranes whereupon they assemble into pores or channels destabilising the membranes leading to cell death. However, specific molecular interactions responsible for antimicrobial activities have yet to be fully understood. Herein we probe such interactions by assessing molecular specific variations in the near-THz 400-40 cm -1 range for defined helical AMP templates in reconstituted phospholipid membranes. In particular, we show that a temperature-dependent spectroscopic analysis, supported by 2D correlative tools, provides direct evidence for the membrane-induced and folding-mediated activity of AMPs. The far-FTIR study offers a direct and information-rich probe of membrane-related antimicrobial interactions.
(© 2022 The Authors. ChemPhysChem published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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