Effect of peripheral iridectomy on VEGF-A and TGF-β levels in rabbit aqueous humour.

Autor: Chew ACY; Singapore National Eye Centre, 11 Third Hospital Avenue, Singapore, 168751, Singapore.; Singapore Eye Research Institute, Singapore, Singapore.; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.; Duke-NUS Medical School Singapore, Singapore, Singapore., Seet LF; Singapore Eye Research Institute, Singapore, Singapore.; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.; Duke-NUS Medical School Singapore, Singapore, Singapore., Chu SWL; Singapore Eye Research Institute, Singapore, Singapore., Lwin NC; Singapore Eye Research Institute, Singapore, Singapore., Wong TT; Singapore National Eye Centre, 11 Third Hospital Avenue, Singapore, 168751, Singapore. tina.wong.t.l@singhealth.com.sg.; Singapore Eye Research Institute, Singapore, Singapore. tina.wong.t.l@singhealth.com.sg.; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore. tina.wong.t.l@singhealth.com.sg.; Duke-NUS Medical School Singapore, Singapore, Singapore. tina.wong.t.l@singhealth.com.sg.
Jazyk: angličtina
Zdroj: BMC ophthalmology [BMC Ophthalmol] 2022 Jan 14; Vol. 22 (1), pp. 23. Date of Electronic Publication: 2022 Jan 14.
DOI: 10.1186/s12886-022-02249-6
Abstrakt: Background: Peripheral iridectomy (PI), routinely performed during glaucoma filtration surgery, may contribute to scarring. This study aims to determine whether PI alters the concentrations of VEGF-A and TGF-β isoforms in the rabbit aqueous humour.
Methods: Anterior chamber paracentesis (ACP) was performed in both eyes of six New Zealand white rabbits, with additional surgical PI performed in the right eyes. Eyes were examined on postoperative days (PODs) 1, 7, 30 and 60 by means of the tonopen, slit-lamp biomicroscopy, and bead-based cytokine assays for TGF-β and VEGF-A concentrations in the aqueous humor.
Results: ACP caused a significant reduction in intraocular pressure (IOP) from mean preoperative 11.47 ± 1.01 mmHg to 5.67 ± 1.63 mmHg on POD 1 while PI did not cause further IOP reduction. Limbal conjunctival vasculature appeared slightly increased on POD 1 in both ACP and PI eyes with PI also causing mild bleeding from damaged iris vessels. Two PI eyes developed fibrinous anterior chamber reaction and/ or peripheral anterior synechiae. Aqueous VEGF-A levels were not significantly different between eyes treated with ACP and PI. Aqueous TGF-β concentrations distributed in the ratio of 4:800:1 for TGF-β1:TGF-β2:TGF-β3 respectively. While aqueous TGF-β2 was not significantly induced by either procedure at any time point, TGF-β1 and TGF-β3 were significantly induced above baseline levels by PI on POD 1.
Conclusion: PI increases the risk of inflammation. The combined induction of aqueous TGF-β1 and TGF-β3 by PI in glaucoma surgery may impact surgery success in glaucoma subtypes sensitive to these isoforms.
(© 2022. The Author(s).)
Databáze: MEDLINE
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