pH-Dependent Self-Assembly of Human Neuropeptide Hormone GnRH into Functional Amyloid Nanofibrils and Hexagonal Phases.
| Autor: | Dharmadana D; School of Health and Biomedical Sciences, RMIT University, VIC 3083, Bundoora, Victoria, Australia.; School of Science, RMIT University, VIC 3001, Melbourne, Victoria, Australia., Reynolds NP; ARC Training Center for Biodevices, Swinburne University, VIC 3122, Melbourne, Victoria, Australia., Conn CE; School of Science, RMIT University, VIC 3001, Melbourne, Victoria, Australia., Valéry C; School of Health and Biomedical Sciences, RMIT University, VIC 3083, Bundoora, Victoria, Australia. |
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| Jazyk: | angličtina |
| Zdroj: | ACS applied bio materials [ACS Appl Bio Mater] 2019 Aug 19; Vol. 2 (8), pp. 3601-3606. Date of Electronic Publication: 2019 Aug 08. |
| DOI: | 10.1021/acsabm.9b00468 |
| Abstrakt: | Gonadotropin-releasing hormone (GnRH) is a short human neuropeptide involved in the regulation of sex hormones. We report that GnRH self-assembles into reversible β-sheet-based nanofibrils, with pH-dependent kinetics. At high concentrations, these nanostructures form arrays arranged in liquid crystalline hexagonal phases. Histidine deprotonation with increasing pH can mediate the formation of β-sheet-based precipitates. Our results are relevant to functional amyloids in general, to the intracellular storage process of GnRH within secretory granules, and to peptide hormone drug delivery. |
| Databáze: | MEDLINE |
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