Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.

Autor: Shan G; Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China. Electronic address: shan_guang@aliyun.com., Huang T; Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China., Tang T; Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China. Electronic address: tang_tian1@yeah.net.
Jazyk: angličtina
Zdroj: Pathology, research and practice [Pathol Res Pract] 2022 Jan; Vol. 229, pp. 153734. Date of Electronic Publication: 2021 Dec 05.
DOI: 10.1016/j.prp.2021.153734
Abstrakt: Clear cell renal cell carcinoma (ccRCC) is recognized as one of the most lethal malignancies among the urological system, with constantly increasing mortality. While the molecular mechanisms underlying ccRCC progression are still poorly understood, the molecular and functional role of lncRNA in multiple diseases has been well demonstrated. In this study, we hypothesized that lncRNA MEG8 might participate in ccRCC development. At first, we found that MEG8 expression was increased in ccRCC tumor tissues and cells. Next, we demonstrated that MEG8 knockdown suppressed cell viability, migration, and invasion in vitro and inhibited tumor growth in vivo. Subsequently, we utilized bioinformatics analysis, ChIP, and luciferase assays, and we found that PLAG1 could transcriptionally regulate MEG8 in ccRCC cells. Furthermore, MEG8 promoted G3BP1 expression to aggravate ccRCC tumorigenic properties through sponging miR-495-3p. Our study identified a novel PLAG1/MEG8/miR-495-3p/G3BP1 network in ccRCC development, which might be a promising direction for developing new diagnoses or therapeutic agents for ccRCC.
(Copyright © 2022 The Authors. Published by Elsevier GmbH.. All rights reserved.)
Databáze: MEDLINE
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