Pseudomonas aeruginosa rhamnolipid micelles deliver toxic metabolites and antibiotics into Staphylococcus aureus .

Autor:	Gdaniec BG; Transplant Infectious Diseases Unit, University Hospitals Geneva, Rue Gabrielle-Perret-Gentil 4, 1205 Geneva, Switzerland.; Department of Microbiology and Molecular Medicine, University of Geneva, 1, Rue Michel Servet, 1211 Geneva, Switzerland.; Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, 1, Rue Michel Servet, 1211 Geneva, Switzerland., Bonini F; Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, 1, Rue Michel Servet, 1211 Geneva, Switzerland., Prodon F; Bioimaging Core Facility, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland., Braschler T; Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, 1, Rue Michel Servet, 1211 Geneva, Switzerland., Köhler T; Transplant Infectious Diseases Unit, University Hospitals Geneva, Rue Gabrielle-Perret-Gentil 4, 1205 Geneva, Switzerland.; Department of Microbiology and Molecular Medicine, University of Geneva, 1, Rue Michel Servet, 1211 Geneva, Switzerland., van Delden C; Transplant Infectious Diseases Unit, University Hospitals Geneva, Rue Gabrielle-Perret-Gentil 4, 1205 Geneva, Switzerland.; Department of Microbiology and Molecular Medicine, University of Geneva, 1, Rue Michel Servet, 1211 Geneva, Switzerland.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2021 Dec 20; Vol. 25 (1), pp. 103669. Date of Electronic Publication: 2021 Dec 20 (Print Publication: 2022).
DOI:	10.1016/j.isci.2021.103669
Abstrakt:	Efficient delivery of toxic compounds to bacterial competitors is essential during interspecies microbial warfare. Rhamnolipids (RLPs) are glycolipids produced by Pseudomonas and Burkholderia species involved in solubilization and uptake of environmental aliphatic hydrocarbons and perform as biosurfactants for swarming motility. Here, we show that RLPs produced by Pseudomonas aeruginosa associate to form micelles. Using high-resolution microscopy, we found that RLP micelles serve as carriers for self-produced toxic compounds, which they deliver to Staphylococcus aureus cells, thereby enhancing and accelerating S. aureus killing. RLPs also potentiated the activity of lincosamide antibiotics, suggesting that RLP micelles may transport not only self-produced but also heterologous compounds to target competing bacterial species. Competing Interests: The authors declare no competing interests. (© 2021 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu