Direct detection of SARS-CoV-2 RNA using high-contrast pH-sensitive dyes.

Autor:	Brown TA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Schaefer KS; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Tsang A; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Yi HA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Grimm JB; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Lemire AL; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Jradi FM; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Kim C; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., McGowan K; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Ritola K; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Armstrong DT; Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-7093., Mostafa HH; Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-7093., Korff W; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Vale RD; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147., Lavis LD; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147.
Jazyk:	angličtina
Zdroj:	Journal of biomolecular techniques : JBT [J Biomol Tech] 2021 Sep; Vol. 32 (3), pp. 121-133.
DOI:	10.7171/jbt.21-3203-007
Abstrakt:	The worldwide coronavirus disease 2019 pandemic has had devastating effects on health, healthcare infrastructure, social structure, and economics. One of the limiting factors in containing the spread of this virus has been the lack of widespread availability of fast, inexpensive, and reliable methods for testing of individuals. Frequent screening for infected and often asymptomatic people is a cornerstone of pandemic management plans. Here, we introduce 2 pH-sensitive "LAMPshade" dyes as novel readouts in an isothermal Reverse Transcriptase Loop-mediated isothermal AMPlification amplification assay for severe acute respiratory syndrome coronavirus 2 RNA. The resulting JaneliaLAMP assay is robust, simple, inexpensive, and has low technical requirements, and we describe its use and performance in direct testing of contrived and clinical samples without RNA extraction. (© 2021 ABRF.)
Databáze:	MEDLINE
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