Novel Cluster AZ Arthrobacter phages Powerpuff, Lego, and YesChef exhibit close functional relationships with Microbacterium phages.

Autor: Kapinos A; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Aghamalian P; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Capehart E; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Alag A; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Angel H; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Briseno E; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Corado Perez B; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Farag E; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Foster H; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Hakim A; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Hernandez-Casas D; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Huang C; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Lam D; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Mendez M; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Min A; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Nguyen N; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Omholt AL; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Ortiz E; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Saldivar LS; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Shannon JA; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Smith R; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Sridhar MV; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Ta A; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Theophilus MC; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Ngo R; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Torres C; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Reddi K; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Freise AC; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America., Moberg Parker J; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Jan 13; Vol. 17 (1), pp. e0262556. Date of Electronic Publication: 2022 Jan 13 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0262556
Abstrakt: Bacteriophages exhibit a vast spectrum of relatedness and there is increasing evidence of close genomic relationships independent of host genus. The variability in phage similarity at the nucleotide, amino acid, and gene content levels confounds attempts at quantifying phage relatedness, especially as more novel phages are isolated. This study describes three highly similar novel Arthrobacter globiformis phages-Powerpuff, Lego, and YesChef-which were assigned to Cluster AZ using a nucleotide-based clustering parameter. Phages in Cluster AZ, Microbacterium Cluster EH, and the former Microbacterium singleton Zeta1847 exhibited low nucleotide similarity. However, their gene content similarity was in excess of the recently adopted Microbacterium clustering parameter, which ultimately resulted in the reassignment of Zeta1847 to Cluster EH. This finding further highlights the importance of using multiple metrics to capture phage relatedness. Additionally, Clusters AZ and EH phages encode a shared integrase indicative of a lysogenic life cycle. In the first experimental verification of a Cluster AZ phage's life cycle, we show that phage Powerpuff is a true temperate phage. It forms stable lysogens that exhibit immunity to superinfection by related phages, despite lacking identifiable repressors typically required for lysogenic maintenance and superinfection immunity. The ability of phage Powerpuff to undergo and maintain lysogeny suggests that other closely related phages may be temperate as well. Our findings provide additional evidence of significant shared phage genomic content spanning multiple actinobacterial host genera and demonstrate the continued need for verification and characterization of life cycles in newly isolated phages.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje