Illuminating the biochemical interaction of antimicrobial few-layer black phosphorus with microbial cells using synchrotron macro-ATR-FTIR.

Autor:	Shaw ZL; School of Engineering, College of Science, Engineering and Health, RMIT University, Melbourne, Victoria 3001, Australia. sumeet.walia@rmit.edu.au., Cheeseman S; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne Victoria 3001, Australia. aaron.elbourne@rmit.edu.au., Huang LZY; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne Victoria 3001, Australia. aaron.elbourne@rmit.edu.au., Penman R; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne Victoria 3001, Australia. aaron.elbourne@rmit.edu.au., Ahmed T; School of Engineering, College of Science, Engineering and Health, RMIT University, Melbourne, Victoria 3001, Australia. sumeet.walia@rmit.edu.au.; Pak-Austria Fachhochschule: Institute of Applied Sciences and Technology, Haripur, 22620, Pakistan., Bryant SJ; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne Victoria 3001, Australia. aaron.elbourne@rmit.edu.au., Bryant G; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne Victoria 3001, Australia. aaron.elbourne@rmit.edu.au., Christofferson AJ; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne Victoria 3001, Australia. aaron.elbourne@rmit.edu.au., Orrell-Trigg R; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne Victoria 3001, Australia. aaron.elbourne@rmit.edu.au., Dekiwadia C; RMIT Microscopy and Microanalysis Facility (RMMF), RMIT University, Melbourne, Victoria 3001, Australia., Truong VK; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne Victoria 3001, Australia. aaron.elbourne@rmit.edu.au., Vongsvivut JP; Infrared Microspectroscopy Beamline, ANSTO Australian Synchrotron, Clayton, Victoria 3168, Australia., Walia S; School of Engineering, College of Science, Engineering and Health, RMIT University, Melbourne, Victoria 3001, Australia. sumeet.walia@rmit.edu.au.; Functional Materials and Microsystems Research Group and MicroNano Research Facility, RMIT University, Melbourne, Victoria 3001, Australia., Elbourne A; School of Science, College of Science, Engineering and Health, RMIT University, Melbourne Victoria 3001, Australia. aaron.elbourne@rmit.edu.au.
Jazyk:	angličtina
Zdroj:	Journal of materials chemistry. B [J Mater Chem B] 2022 Sep 28; Vol. 10 (37), pp. 7527-7539. Date of Electronic Publication: 2022 Sep 28.
DOI:	10.1039/d1tb02575a
Abstrakt:	In the fight against drug-resistant pathogenic bacterial and fungal cells, low-dimensional materials are emerging as a promising alternative treatment method. Specifically, few-layer black phosphorus (BP) has demonstrated its effectiveness against a wide range of pathogenic bacterial and fungal cells with studies suggesting low cytotoxicity towards healthy mammalian cells. However, the antimicrobial mechanism of action of BP is not well understood. Before new applications for this material can be realised, further in-depth investigations are required. In this work, the biochemical interaction between BP and a series of microbial cells is investigated using a variety of microscopy and spectroscopy techniques to provide a greater understanding of the antimicrobial mechanism. Synchrotron macro-attenuated total reflection-Fourier transform infrared (ATR-FTIR) micro-spectroscopy is used to elucidate the chemical changes occurring outside and within the cell of interest after exposure to BP nanoflakes. The ATR-FTIR data, coupled with high-resolution microscopy, reveals major physical and bio-chemical changes to the phospholipids and amide I and II proteins, as well as minor chemical changes to the structural polysaccharides and nucleic acids when compared to untreated cells. These changes can be attributed to the physical interaction of the BP nanoflakes with the cell membranes, combined with the oxidative stress induced by the degradation of the BP nanoflakes. This study provides insight into the biochemical interaction of BP nanoflakes with microbial cells, allowing for a better understanding of the antimicrobial mechanism of action that will be important for the next generation of applications such as implant coatings, wound dressings, or medical surfaces.
Databáze:	MEDLINE
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