Heterologous expression of four recombinant toxins from Panamanian scorpions of the genus Tityus and Centruroides for production of antivenom.

Autor:	Salazar MH; Centro de Investigación e Información de Medicamentos y Tóxicos (CIIMET), Facultad de Medicina, Universidad de Panamá, Ciudad de Panamá, Panamá.; Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá, Ciudad de Panamá, Panamá.; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México., Clement H; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México., Corrales-García LL; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.; Departamento de Alimentos, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, AA 1226, Medellín, 050010, Colombia., Sánchez J; Centro de Investigación e Información de Medicamentos y Tóxicos (CIIMET), Facultad de Medicina, Universidad de Panamá, Ciudad de Panamá, Panamá.; Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá, Ciudad de Panamá, Panamá., Cleghorn J; Centro de Investigación e Información de Medicamentos y Tóxicos (CIIMET), Facultad de Medicina, Universidad de Panamá, Ciudad de Panamá, Panamá., Zamudio F; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México., Possani LD; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México., Acosta H; Centro de Investigación e Información de Medicamentos y Tóxicos (CIIMET), Facultad de Medicina, Universidad de Panamá, Ciudad de Panamá, Panamá., Corzo G; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
Jazyk:	angličtina
Zdroj:	Toxicon: X [Toxicon X] 2021 Dec 28; Vol. 13, pp. 100090. Date of Electronic Publication: 2021 Dec 28 (Print Publication: 2022).
DOI:	10.1016/j.toxcx.2021.100090
Abstrakt:	Background: The development of more effective antivenoms remains a necessity for countries where scorpionism is a public health problem. Also, the regionalization of antivenoms may be important for some countries with special scorpionism characteristics. Objective: Production of antibodies capable of neutralizing the lethal effect of the venom of three scorpion species from Panama. Methods: The primary structures of two neurotoxins from T. pachyurus, one from T. cerroazul and another from C. bicolor were elucidated using N-terminal amino acid degradation and Sanger gene cloned sequencing. The obtained mRNA transcripts were cloned and expressed using E. coli vectors. Different bacterial expression conditions were tested and the best culture conditions for each expressed protein is reported. The expressed scorpion toxins were purified by chromatographic methods and used as immunogens in rabbits. Results: The antibodies produced under the reported immunization scheme show better neutralization (ED 50 ) than other reported commercial antivenoms used to neutralize similar species scorpion venoms under similar LD 50 conditions. Conclusion: The information reported here shows the proof of concept for selecting recombinant immunogens with the ability to produce antibodies for neutralizing the lethal effects of the most important medical species of scorpions in Panama. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Author(s).)
Databáze:	MEDLINE
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