Detection of invasive Trichosporon asahii in patient blood by a fungal PCR array.

Autor:	Weber JK; Institute for Medical Microbiology and Hospital Hygiene, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Scharf S; Institute for Medical Microbiology and Hospital Hygiene, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Walther G; German National Reference Centre for Invasive Fungal Infections, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany., Flüh G; Institute for Medical Microbiology and Hospital Hygiene, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.; Labor Dr. Wisplinghoff, Cologne, Germany., MacKenzie CR; Institute for Medical Microbiology and Hospital Hygiene, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Kondakci M; Department of Haematology, Oncology and Clinical Immunology, University Hospital Düsseldorf, Düsseldorf, Germany., Henrich B; Institute for Medical Microbiology and Hospital Hygiene, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Kohns Vasconcelos M; Institute for Medical Microbiology and Hospital Hygiene, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Jazyk:	angličtina
Zdroj:	Access microbiology [Access Microbiol] 2021 Dec 03; Vol. 3 (12), pp. 000285. Date of Electronic Publication: 2021 Dec 03 (Print Publication: 2021).
DOI:	10.1099/acmi.0.000285
Abstrakt:	Rare invasive fungal infections are increasingly emerging in hosts with predisposing factors such as immunodeficiency. Their timely diagnosis remains difficult, as their clinical picture may initially mimic infections with more common fungal species and species identification may be difficult with routine methods or may require time-consuming subcultures. This often results in ineffective drug administration and fatal outcomes. We report on a patient in their early twenties with mixed cellularity classical Hodgkin lymphoma with a disseminated Trichosporon asahii ( T. asahii ) infection. Even though pathogen detection and identification was possible via the standard procedure consisting of culture followed by matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF) mass spectrometry, the patient passed away in the course of multi organ failure. Herein, we report on a retrospectively applied experimental diagnostic fungal PCR-analysis used on an EDTA blood sample and consisting of two pan-fungal reactions and seven branch-specific reactions. Regarding invasive T. asahii infection, this PCR array could considerably shorten time to diagnosis and switch to a targeted therapy with triazoles. Competing Interests: The authors declare that there are no conflicts of interest. (© 2021 The Authors.)
Databáze:	MEDLINE
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