Solid Phase Peptide Synthesis on Chitosan Thin Films.

Autor:	Katan T; Institute of Chemistry and Technology of Biobased Systems (IBioSys), Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria., Kargl R; Institute of Chemistry and Technology of Biobased Systems (IBioSys), Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria., Mohan T; Institute of Chemistry and Technology of Biobased Systems (IBioSys), Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria., Steindorfer T; Institute of Chemistry and Technology of Biobased Systems (IBioSys), Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria., Mozetič M; Department of Surface Engineering, Jožef Stefan Institute (IJS), Jamova 39, 1000 Ljubljana, Slovenia., Kovač J; Department of Surface Engineering, Jožef Stefan Institute (IJS), Jamova 39, 1000 Ljubljana, Slovenia., Stana Kleinschek K; Institute of Chemistry and Technology of Biobased Systems (IBioSys), Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria.
Jazyk:	angličtina
Zdroj:	Biomacromolecules [Biomacromolecules] 2022 Mar 14; Vol. 23 (3), pp. 731-742. Date of Electronic Publication: 2022 Jan 13.
DOI:	10.1021/acs.biomac.1c01155
Abstrakt:	Stable chitosan thin films can be promising substrates for creating nanometric peptide-bound polyglucosamine layers. Those are of scientific interest since they can have certain structural similarities to bacterial peptidoglycans. Such films were deposited by spin coating from chitosan solutions and modified by acetylation and N -protected amino acids. The masses of deposited materials and their stability in aqueous solutions at different pH values and water interaction were determined with a quartz crystal microbalance with dissipation (QCM-D). The evolution of the surface composition was followed by X-ray photoelectron (XPS) and attenuated total reflectance infrared (ATR-IR) spectroscopy. Morphological changes were measured by atomic force microscopy (AFM), while the surface wettability was monitored by by static water contact angle measurements. The combination of the characterization techniques enabled an insight into the surface chemistry for each treatment step and confirmed the acetylation and coupling of N -protected glycine peptides. The developed procedures are seen as first steps toward preparing thin layers of acetylated chitin, potentially imitating the nanometric peptide substituted glycan layers found in bacterial cell walls.
Databáze:	MEDLINE
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