Strain specific motility patterns and surface adhesion of virulent and probiotic Escherichia coli.

Autor: Abdulkadieva MM; Department of Dusty Plasma, Joint Institute for High Temperatures, Russian Academy of Sciences, Moscow, Russia.; Peoples' Friendship, University of Russia (RUDN University), Moscow, Russia., Sysolyatina EV; Laboratory of Ecology of Pathogenic Bacteria, N. F. Gamaleya National Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia. elena.sysolyatina84@gmail.com., Vasilieva EV; Department of Dusty Plasma, Joint Institute for High Temperatures, Russian Academy of Sciences, Moscow, Russia.; Laboratory of Ecology of Pathogenic Bacteria, N. F. Gamaleya National Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia., Gusarov AI; National Research University 'Moscow Power Engineering Institute', Moscow, Russia., Domnin PA; Laboratory of Ecology of Pathogenic Bacteria, N. F. Gamaleya National Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia., Slonova DA; Laboratory of Molecular Microbiology, Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, Russia.; Laboratory of Molecular Immunology, Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Russian Ministry of Health, Moscow, Russia., Stanishevskiy YM; Peoples' Friendship, University of Russia (RUDN University), Moscow, Russia., Vasiliev MM; Department of Dusty Plasma, Joint Institute for High Temperatures, Russian Academy of Sciences, Moscow, Russia., Petrov OF; Department of Dusty Plasma, Joint Institute for High Temperatures, Russian Academy of Sciences, Moscow, Russia., Ermolaeva SA; Laboratory of Ecology of Pathogenic Bacteria, N. F. Gamaleya National Research Centre of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jan 12; Vol. 12 (1), pp. 614. Date of Electronic Publication: 2022 Jan 12.
DOI: 10.1038/s41598-021-04592-y
Abstrakt: Bacterial motility provides the ability for bacterial dissemination and surface exploration, apart from a choice between surface colonisation and further motion. In this study, we characterised the movement trajectories of pathogenic and probiotic Escherichia coli strains (ATCC43890 and M17, respectively) at the landing stage (i.e., leaving the bulk and approaching the surface) and its correlation with adhesion patterns and efficiency. A poorly motile strain JM109 was used as a control. Using specially designed and manufactured microfluidic chambers, we found that the motion behaviour near surfaces drastically varied between the strains, correlating with adhesion patterns. We consider two bacterial strategies for effective surface colonisation: horizontal and vertical, based on the obtained results. The horizontal strategy demonstrated by the M17 strain is characterised by collective directed movements within the horizontal layer during a relatively long period and non-uniform adhesion patterns, suggesting co-dependence of bacteria in the course of adhesion. The vertical strategy demonstrated by the pathogenic ATCC43890 strain implies the individual movement of bacteria mainly in the vertical direction, a faster transition from bulk to near-surface swimming, and independent bacterial behaviour during adhesion, providing a uniform distribution over the surface.
(© 2022. The Author(s).)
Databáze: MEDLINE
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