NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis.

Autor: Bieluszewski T; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland., Sura W; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland., Dziegielewski W; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland., Bieluszewska A; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland., Lachance C; Laval University Cancer Research Center, CHU de Québec-UL Research Center-Oncology Division, Quebec City, QC, G1R 3S3, Canada., Kabza M; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland., Szymanska-Lejman M; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland., Abram M; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland., Wlodzimierz P; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland., De Winne N; VIB-UGent Center for Plant Systems Biology, 9052, Ghent, Belgium., De Jaeger G; VIB-UGent Center for Plant Systems Biology, 9052, Ghent, Belgium., Sadowski J; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland., Côté J; Laval University Cancer Research Center, CHU de Québec-UL Research Center-Oncology Division, Quebec City, QC, G1R 3S3, Canada. jacques.cote@crchudequebec.ulaval.ca., Ziolkowski PA; Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614, Poznan, Poland. pzio@amu.edu.pl.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Jan 12; Vol. 13 (1), pp. 277. Date of Electronic Publication: 2022 Jan 12.
DOI: 10.1038/s41467-021-27882-5
Abstrakt: Nucleosomal acetyltransferase of H4 (NuA4) is an essential transcriptional coactivator in eukaryotes, but remains poorly characterized in plants. Here, we describe Arabidopsis homologs of the NuA4 scaffold proteins Enhancer of Polycomb-Like 1 (AtEPL1) and Esa1-Associated Factor 1 (AtEAF1). Loss of AtEAF1 results in inhibition of growth and chloroplast development. These effects are stronger in the Atepl1 mutant and are further enhanced by loss of Golden2-Like (GLK) transcription factors, suggesting that NuA4 activates nuclear plastid genes alongside GLK. We demonstrate that AtEPL1 is necessary for nucleosomal acetylation of histones H4 and H2A.Z by NuA4 in vitro. These chromatin marks are diminished genome-wide in Atepl1, while another active chromatin mark, H3K9 acetylation (H3K9ac), is locally enhanced. Expression of many chloroplast-related genes depends on NuA4, as they are downregulated with loss of H4ac and H2A.Zac. Finally, we demonstrate that NuA4 promotes H2A.Z deposition and by doing so prevents spurious activation of stress response genes.
(© 2022. The Author(s).)
Databáze: MEDLINE
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