Identification of human gene research articles with wrongly identified nucleotide sequences.

Autor:	Park Y; Faculty of Medicine and Health, The University of Sydney, Sydney, Australia., West RA; Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.; Children's Cancer Research Unit, Kids Research, The Children's Hospital at Westmead, Westmead, Australia., Pathmendra P; Faculty of Medicine and Health, The University of Sydney, Sydney, Australia., Favier B; Université Grenoble Alpes, Translationnelle et Innovation en Médecine et Complexité, Grenoble, France., Stoeger T; Successful Clinical Response in Pneumonia Therapy Systems Biology Center, Northwestern University, Evanston, IL, USA.; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Center for Genetic Medicine, Northwestern University School of Medicine, Chicago, IL, USA., Capes-Davis A; Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.; CellBank Australia, Children's Medical Research Institute, Westmead, Australia., Cabanac G; Computer Science Department, Institut de Recherche en Informatique de Toulouse, Unité Mixte de Recherche 5505 Centre National de la Recherche Scientifique (CNRS), University of Toulouse, Toulouse, France., Labbé C; Université Grenoble Alpes, CNRS, Grenoble INP, Laboratoire d'Informatique de Grenoble, Grenoble, France., Byrne JA; Faculty of Medicine and Health, The University of Sydney, Sydney, Australia jennifer.byrne@health.nsw.gov.au.; New South Wales Health Statewide Biobank, New South Wales Health Pathology, Camperdown, Australia.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2022 Jan 12; Vol. 5 (4). Date of Electronic Publication: 2022 Jan 12 (Print Publication: 2022).
DOI:	10.26508/lsa.202101203
Abstrakt:	Nucleotide sequence reagents underpin molecular techniques that have been applied across hundreds of thousands of publications. We have previously reported wrongly identified nucleotide sequence reagents in human research publications and described a semi-automated screening tool Seek & Blastn to fact-check their claimed status. We applied Seek & Blastn to screen >11,700 publications across five literature corpora, including all original publications in Gene from 2007 to 2018 and all original open-access publications in Oncology Reports from 2014 to 2018. After manually checking Seek & Blastn outputs for >3,400 human research articles, we identified 712 articles across 78 journals that described at least one wrongly identified nucleotide sequence. Verifying the claimed identities of >13,700 sequences highlighted 1,535 wrongly identified sequences, most of which were claimed targeting reagents for the analysis of 365 human protein-coding genes and 120 non-coding RNAs. The 712 problematic articles have received >17,000 citations, including citations by human clinical trials. Given our estimate that approximately one-quarter of problematic articles may misinform the future development of human therapies, urgent measures are required to address unreliable gene research articles. (© 2022 Park et al.)
Databáze:	MEDLINE
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