Mannose-Coated Fluorescent Lipid Microparticles for Specific Cellular Targeting and Internalization via Glycoreceptor-Induced Phagocytosis.

Autor:	Dumat B; Laboratoire des Biomolécules, LBM, Département de Chimie, École Normale Supérieure, CNRS, PSL University, Sorbonne Université, Paris 75005, France., Montel L; PASTEUR, Département de Chimie, École Normale Supérieure, CNRS, PSL University, Sorbonne Université, Paris 75005, France.; Institut Pierre-Gilles de Gennes pour la Microfluidique, Paris 75005, France., Pinon L; PASTEUR, Département de Chimie, École Normale Supérieure, CNRS, PSL University, Sorbonne Université, Paris 75005, France.; Institut Pierre-Gilles de Gennes pour la Microfluidique, Paris 75005, France., Matton P; Laboratoire des Biomolécules, LBM, Département de Chimie, École Normale Supérieure, CNRS, PSL University, Sorbonne Université, Paris 75005, France., Cattiaux L; Laboratoire des Biomolécules, LBM, Département de Chimie, École Normale Supérieure, CNRS, PSL University, Sorbonne Université, Paris 75005, France., Fattaccioli J; PASTEUR, Département de Chimie, École Normale Supérieure, CNRS, PSL University, Sorbonne Université, Paris 75005, France.; Institut Pierre-Gilles de Gennes pour la Microfluidique, Paris 75005, France., Mallet JM; Laboratoire des Biomolécules, LBM, Département de Chimie, École Normale Supérieure, CNRS, PSL University, Sorbonne Université, Paris 75005, France.
Jazyk:	angličtina
Zdroj:	ACS applied bio materials [ACS Appl Bio Mater] 2019 Nov 18; Vol. 2 (11), pp. 5118-5126. Date of Electronic Publication: 2019 Nov 04.
DOI:	10.1021/acsabm.9b00793
Abstrakt:	In this work, we report on the development of mannose-coated fluorescent lipid microparticles to study the role of C-type lectin membrane receptors in phagocytosis. The micrometric droplets of soybean oil-in-water emulsion were functionalized with a tailor-made fluorescent mannolipid. The amphiphilic ligand was built from a mannose unit, a lipid C11 spacer, and a naphthalimide fluorophore. The functionalization of the droplets was monitored by fluorescence microscopy as well as their interaction with concanavalin A, which was used as a model lectin in vitro . The use of a monovalent ligand on the surface of emulsion droplets yielded particles with an affinity approximately 40 times higher than that of free mannose. In cellulo , the coated droplets were shown to be specifically internalized by macrophages in a receptor-dependent phagocytic pathway. The naked droplets, on the other hand, displayed very little internalization because of their low immunogenicity. This work thus brings evidence that C-type lectin membrane receptors may act as phagocytic receptors. The functionalization of the droplets with the tailored amphiphilic fluorescent ligand also provides insights into the development of organic fluorescent particles that may prove useful for developing targeted imaging and delivery tools.
Databáze:	MEDLINE
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