| Autor: |
Fakhardo AF; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Anastasova EI; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Gabdullina SR; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Solovyeva AS; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Saparova VB; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Chrishtop VV; Ivanovo State Medical Academy, Ivanovo 153012, Russia., Koshevaya ED; Institute of Chemistry, FRC Komi SC UB RAS, Syktyvkar 167982, Russia., Krivoshapkina EF; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Krivoshapkin PV; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Kiselev GO; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Kalikina PA; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Koshel EI; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia., Shtil AA; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia.; Blokhin National Medical Research Center of Oncology, Moscow 115478, Russia., Vinogradov VV; Laboratory of Solution Chemistry of Advanced Materials and Technology, ITMO University, St. Petersburg 197101, Russia. |
| Abstrakt: |
Nanostructured drugs are being approved for clinical use, although there is a serious deficit of systematic studies of these materials. Data on toxicity of nanoparticles (NPs) can vary due to different methods of preparation, size, and shape. We investigated the toxicity against cultured human cells, the acute toxicity in mice, and the influence on conjugative transfer of antibiotic resistance genes of clinically relevant NPs such as TiO 2 , ZrO 2 , HfO 2 , Ta 2 O 5 , Fe 3 O 4 , and AlOOH. NPs were synthesized as aqueous sols by the same method in aqueous solution, with almost identical size 2-10 nm. None of these NPs was cytotoxic at concentrations compatible with water solubility. Furthermore, TiO 2 , HfO 2 , Ta 2 O 5 , Fe 3 O 4 , and AlOOH were not toxic to mice after oral administration. However, ZrO 2 showed rather high toxicity, with LD 50 2277.8 mg/kg. Experiments with plasmid transfer between bacteria demonstrated that AlOOH NPs were the most hazardous since this material promoted the emergence of resistance to antibiotics. Thus, although our metal oxide NPs are largely non-toxic, their properties may differ in specific biological situations. |
