Detection of NS1 protein from dengue virus in excreta and homogenates of wild-caught Aedes aegypti mosquitoes using monoclonal antibodies.

Autor:	Rodriguez MC; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Av. Universidad 655, C.P. 62100 Cuernavaca, Morelos, Mexico., Cime-Castillo J; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Av. Universidad 655, C.P. 62100 Cuernavaca, Morelos, Mexico., Argotte-Ramos R; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Av. Universidad 655, C.P. 62100 Cuernavaca, Morelos, Mexico., Vargas V; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Av. Universidad 655, C.P. 62100 Cuernavaca, Morelos, Mexico., Correa-Morales F; Dirección del Programa de Enfermedades Transmitidas por Vector, Centro Nacional de Programas Preventivos y Control de Enfermedades, Secretaría de Salud México, Benjamin Franklin 132, C.P. 11800 Ciudad de México, Mexico., Sánchez-Tejeda G; Dirección del Programa de Enfermedades Transmitidas por Vector, Centro Nacional de Programas Preventivos y Control de Enfermedades, Secretaría de Salud México, Benjamin Franklin 132, C.P. 11800 Ciudad de México, Mexico., Lanz-Mendoza H; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Av. Universidad 655, C.P. 62100 Cuernavaca, Morelos, Mexico.
Jazyk:	angličtina
Zdroj:	Pathogens and disease [Pathog Dis] 2022 Feb 09; Vol. 80 (1).
DOI:	10.1093/femspd/ftac002
Abstrakt:	Dengue fever is one of the most devastating infectious diseases worldwide. Development of methods for dengue virus (DENV) detection in mosquitoes to assess prevalence as a preliminary screen for entomological surveillance in endemic regions of DENV will certainly contribute to the control of the disease. A monoclonal antibody against the NS1 (nonstructural protein 1) viral protein was generated using recombinant NS1 protein and used to detect and analyze DENV in both excreta and total homogenates from Aedes aegypti mosquitoes. Results demonstrated expression of NS1 in excreta of DENV laboratory-infected mosquitoes and homogenates from field mosquitoes infected with DENV. The immunodetection method reported here represents a first-line strategy for assessing the prevalence of DENV in mosquitoes, for entomological surveillance in endemic regions of dengue. Detection of DENV prevalence in field mosquitoes could have an impact on vector surveillance measures to interrupt dengue transmission. (© The Author(s) 2022. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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