Hybridization chain reaction enables a unified approach to multiplexed, quantitative, high-resolution immunohistochemistry and in situ hybridization.
| Autor: | Schwarzkopf M; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Liu MC; Molecular Instruments, Los Angeles, CA 90041, USA., Schulte SJ; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Ives R; Molecular Instruments, Los Angeles, CA 90041, USA., Husain N; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Choi HMT; Molecular Instruments, Los Angeles, CA 90041, USA., Pierce NA; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.; Division of Engineering & Applied Science, California Institute of Technology, Pasadena, CA 91125, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Development (Cambridge, England) [Development] 2021 Nov 15; Vol. 148 (22). Date of Electronic Publication: 2021 Nov 18. |
| DOI: | 10.1242/dev.199847 |
| Abstrakt: | RNA in situ hybridization based on the mechanism of the hybridization chain reaction (HCR) enables multiplexed, quantitative, high-resolution RNA imaging in highly autofluorescent samples, including whole-mount vertebrate embryos, thick brain slices and formalin-fixed paraffin-embedded tissue sections. Here, we extend the benefits of one-step, multiplexed, quantitative, isothermal, enzyme-free HCR signal amplification to immunohistochemistry, enabling accurate and precise protein relative quantitation with subcellular resolution in an anatomical context. Moreover, we provide a unified framework for simultaneous quantitative protein and RNA imaging with one-step HCR signal amplification performed for all target proteins and RNAs simultaneously. Competing Interests: Competing interests The authors declare competing financial interests in the form of patents, pending patent applications and the startup company Molecular Instruments. (© 2021. Published by The Company of Biologists Ltd.) |
| Databáze: | MEDLINE |
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