SARS-CoV-2 N Gene G29195T Point Mutation May Affect Diagnostic Reverse Transcription-PCR Detection.

Autor:	Ko KKK; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore.; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore.; Genome Institute of Singapore, Genome Institute of Singapore, A*STAR, Singapore.; Yong Loo Lin School of Medicine, National University of Singapore, Singapore.; Duke-NUS Medical School, National University of Singapore, Singapore., Abdul Rahman NB; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore.; Duke-NUS Medical School, National University of Singapore, Singapore., Tan SYL; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore.; Duke-NUS Medical School, National University of Singapore, Singapore., Chan KXL; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore., Goh SS; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore., Sim JHC; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore.; Duke-NUS Medical School, National University of Singapore, Singapore., Lim KL; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore., Tan WL; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore., Chan KS; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore.; Duke-NUS Medical School, National University of Singapore, Singapore., Oon LLE; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore.; Duke-NUS Medical School, National University of Singapore, Singapore., Nagarajan N; Genome Institute of Singapore, Genome Institute of Singapore, A*STAR, Singapore.; Yong Loo Lin School of Medicine, National University of Singapore, Singapore., Suphavilai C; Genome Institute of Singapore, Genome Institute of Singapore, A*STAR, Singapore.
Jazyk:	angličtina
Zdroj:	Microbiology spectrum [Microbiol Spectr] 2022 Feb 23; Vol. 10 (1), pp. e0222321. Date of Electronic Publication: 2022 Jan 12.
DOI:	10.1128/spectrum.02223-21
Abstrakt:	Rapid onsite whole-genome sequencing of two suspected severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) N gene diagnostic escape samples revealed a previously unreported N gene point mutation at genome position 29195. Because the G29195T mutation occurs within a region probed by a commonly referenced U.S. CDC N gene reverse transcription (RT)-PCR assay, we hypothesize that the G29195T mutation rendered the N gene target of a proprietary commercial assay undetectable. The putative diagnostic escape G29195T mutation demonstrates the need for nearly real-time surveillance, as emergence of a novel SARS-CoV-2 variant with the potential to escape diagnostic tests continues to be a threat. IMPORTANCE Accurate diagnostic detection of SARS-CoV-2 currently depends on the large-scale deployment of RT-PCR assays. SARS-CoV-2 RT-PCR assays target predetermined regions in the viral genomes by complementary binding of primers and probes to nucleic acid sequences in the clinical samples. Potential diagnostic escapes, such as those of clinical samples harboring the G29195T mutation, may result in false-negative SARS-CoV-2 RT-PCR results. The rapid detection and sharing of potential diagnostic escapes are essential for diagnostic laboratories and manufacturers around the world, to optimize their assays as SARS-CoV-2 continues to evolve.
Databáze:	MEDLINE
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