Evaluation of two artificial infection methods of live ticks as tools for studying interactions between tick-borne viruses and their tick vectors.

Autor: Migné CV; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.; INRAE, Anses, Ecole Nationale Vétérinaire d'Alfort, UMR VIROLOGIE, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France., Hönig V; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic.; Emerging Viral Diseases Research Group, Veterinary Research Institute, Hudcova 296/70, 621 00, Brno, Czech Republic., Bonnet SI; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.; Functional Genetics of Infectious Diseases Unit, Institut Pasteur, CNRS UMR 2000, Université de Paris, 75015, Paris, France., Palus M; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic.; Emerging Viral Diseases Research Group, Veterinary Research Institute, Hudcova 296/70, 621 00, Brno, Czech Republic., Rakotobe S; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France., Galon C; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France., Heckmann A; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France., Vyletova E; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic.; Faculty of Agriculture and Faculty of Science, University of South Bohemia, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic., Devillers E; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France., Attoui H; INRAE, Anses, Ecole Nationale Vétérinaire d'Alfort, UMR VIROLOGIE, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France., Ruzek D; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic.; Emerging Viral Diseases Research Group, Veterinary Research Institute, Hudcova 296/70, 621 00, Brno, Czech Republic., Moutailler S; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France. sara.moutailler@anses.fr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jan 11; Vol. 12 (1), pp. 491. Date of Electronic Publication: 2022 Jan 11.
DOI: 10.1038/s41598-021-04498-9
Abstrakt: Up to 170 tick-borne viruses (TBVs) have been identified to date. However, there is a paucity of information regarding TBVs and their interaction with respective vectors, limiting the development of new effective and urgently needed control methods. To overcome this gap of knowledge, it is essential to reproduce transmission cycles under controlled laboratory conditions. In this study we assessed an artificial feeding system (AFS) and an immersion technique (IT) to infect Ixodes ricinus ticks with tick-borne encephalitis (TBE) and Kemerovo (KEM) virus, both known to be transmitted predominantly by ixodid ticks. Both methods permitted TBEV acquisition by ticks and we further confirmed virus trans-stadial transmission and onward transmission to a vertebrate host. However, only artificial feeding system allowed to demonstrate both acquisition by ticks and trans-stadial transmission for KEMV. Yet we did not observe transmission of KEMV to mice (IFNAR -/- or BALB/c). Artificial infection methods of ticks are important tools to study tick-virus interactions. When optimally used under laboratory settings, they provide important insights into tick-borne virus transmission cycles.
(© 2022. The Author(s).)
Databáze: MEDLINE
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