| Autor: |
Lea RG; University of Nottingham, Sutton Bonington Campus, Loughborough, UK., Mandon-Pépin B; Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France., Loup B; Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France., Poumerol E; Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France., Jouneau L; Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France., Egbowon BF; University of Nottingham, Sutton Bonington Campus, Loughborough, UK., Bowden A; University of Nottingham, Sutton Bonington Campus, Loughborough, UK., Cotinot C; Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, France., Purdie L; University of Nottingham, Sutton Bonington Campus, Loughborough, UK., Zhang Z; James Hutton Institute, Craigebuckler, Aberdeen, UK., Fowler PA; University of Aberdeen, Institute of Medical Sciences, Aberdeen, UK., Sinclair KD; University of Nottingham, Sutton Bonington Campus, Loughborough, UK. |
| Abstrakt: |
Exposure of the fetal testis to numerous individual environmental chemicals (ECs) is frequently associated with dysregulated development, leading to impaired adult reproductive competence. However, 'real-life' exposure involves complex mixtures of ECs. Here we test the consequences, for the male fetus, of exposing pregnant ewes to EC mixtures derived from pastures treated with biosolids fertiliser (processed human sewage). Fetal testes from continuously exposed ewes were either unaffected at day 80 or exhibited a reduced area of testis immunostained for CYP17A1 protein at day 140. Fetal testes from day 140 pregnant ewes that were exposed transiently for 80-day periods during early (0-80 days), mid (30-110 days), or late (60-140 days) pregnancy had fewer Sertoli cells and reduced testicular area stained for CYP17A1. Male fetuses from ewes exposed during late pregnancy also exhibited reduced fetal body, adrenal and testis mass, anogenital distance, and lowered testosterone; collectively indicative of an anti-androgenic effect. Exposure limited to early gestation induced more testis transcriptome changes than observed for continuously exposed day 140 fetuses. These data suggest that a short period of EC exposure does not allow sufficient time for the testis to adapt. Consequently, testicular transcriptomic changes induced during the first 80 days of gestation may equate with phenotypic effects observed at day 140. In contrast, relatively fewer changes in the testis transcriptome in fetuses exposed continuously to ECs throughout gestation are associated with less severe consequences. Unless corrected by or during puberty, these differential effects would predictably have adverse outcomes for adult testicular function and fertility. |
