An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos.

Autor:	Jevtić P; Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA., Elliott KW; Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH 03824, USA., Watkins SE; Department of Chemistry, University of Wyoming, Laramie, WY 82071, USA., Sreter JA; Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH 03824, USA., Jovic K; Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH 03824, USA., Lehner IB; Department of Chemistry, Keene State College, Keene, NH 03435, USA., Baures PW; Department of Chemistry, Keene State College, Keene, NH 03435, USA., Tsavalas JG; Department of Chemistry, University of New Hampshire, Durham, NH 03824, USA., Levy DL; Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA., Varga K; Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH 03824, USA.
Jazyk:	angličtina
Zdroj:	The Journal of experimental biology [J Exp Biol] 2022 Feb 15; Vol. 225 (4). Date of Electronic Publication: 2022 Feb 15.
DOI:	10.1242/jeb.243662
Abstrakt:	Cryoprotection is of interest in many fields of research, necessitating a greater understanding of different cryoprotective agents. Antifreeze proteins have been identified that have the ability to confer cryoprotection in certain organisms. Antifreeze proteins are an evolutionary adaptation that contributes to the freeze resistance of certain fish, insects, bacteria and plants. These proteins adsorb to an ice crystal's surface and restrict its growth within a certain temperature range. We investigated the ability of an antifreeze protein from the desert beetle Anatolica polita, ApAFP752, to confer cryoprotection in the frog Xenopus laevis. Xenopus laevis eggs and embryos microinjected with ApAFP752 exhibited reduced damage and increased survival after a freeze-thaw cycle in a concentration-dependent manner. We also demonstrate that ApAFP752 localizes to the plasma membrane in eggs and embryonic blastomeres and is not toxic for early development. These studies show the potential of an insect antifreeze protein to confer cryoprotection in amphibian eggs and embryos. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2022. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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