CCR2 promotes monocyte recruitment and intestinal inflammation in mice lacking the interleukin-10 receptor.

Autor: El Sayed S; Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA, 02420, USA.; Faculty of Veterinary Medicine, Department of Microbiology, Zagazig University, Zagazig, Ash Sharkia, Egypt., Patik I; Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA, 02420, USA., Redhu NS; Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA, 02420, USA.; Morphic Therapeutic, Waltham, MA, USA., Glickman JN; Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA., Karagiannis K; Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, USA., El Naenaeey ESY; Faculty of Veterinary Medicine, Department of Microbiology, Zagazig University, Zagazig, Ash Sharkia, Egypt., Elmowalid GA; Faculty of Veterinary Medicine, Department of Microbiology, Zagazig University, Zagazig, Ash Sharkia, Egypt., Abd El Wahab AM; Faculty of Veterinary Medicine, Department of Microbiology, Zagazig University, Zagazig, Ash Sharkia, Egypt., Snapper SB; Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA, 02420, USA.; Division of Gastroenterology, Brigham and Women's Hospital, Boston, MA, USA., Horwitz BH; Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA, 02420, USA. Bruce.Horwitz@childrens.harvard.edu.; Division of Emergency Medicine, Boston Children's Hospital, Boston, MA, USA. Bruce.Horwitz@childrens.harvard.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jan 10; Vol. 12 (1), pp. 452. Date of Electronic Publication: 2022 Jan 10.
DOI: 10.1038/s41598-021-04098-7
Abstrakt: Macrophages are a heterogeneous population of mononuclear phagocytes abundantly distributed throughout the intestinal compartments that adapt to microenvironmental specific cues. In adult mice, the majority of intestinal macrophages exhibit a mature phenotype and are derived from blood monocytes. In the steady-state, replenishment of these cells is reduced in the absence of the chemokine receptor CCR2. Within the intestine of mice with colitis, there is a marked increase in the accumulation of immature macrophages that demonstrate an inflammatory phenotype. Here, we asked whether CCR2 is necessary for the development of colitis in mice lacking the receptor for IL10. We compared the development of intestinal inflammation in mice lacking IL10RA or both IL10RA and CCR2. The absence of CCR2 interfered with the accumulation of immature macrophages in IL10R-deficient mice, including a novel population of rounded submucosal Iba1 + cells, and reduced the severity of colitis in these mice. In contrast, the absence of CCR2 did not reduce the augmented inflammatory gene expression observed in mature intestinal macrophages isolated from mice lacking IL10RA. These data suggest that both newly recruited CCR2-dependent immature macrophages and CCR2-independent residual mature macrophages contribute to the development of intestinal inflammation observed in IL10R-deficient mice.
(© 2022. The Author(s).)
Databáze: MEDLINE
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