Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies.

Autor: Jeyaprakasam NK; Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia., Low VL; Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya, Kuala Lumpur, Malaysia., Liew JWK; Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.; Environmental Health Institute, National Environment Agency, Singapore, Singapore., Pramasivan S; Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia., Wan-Sulaiman WY; Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia., Saeung A; Department of Parasitology, Faculty of Medicine, Center of Insect Vector Study, Chiang Mai University, Chiang Mai, Thailand., Vythilingam I; Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia. indrav@um.edu.my.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jan 10; Vol. 12 (1), pp. 354. Date of Electronic Publication: 2022 Jan 10.
DOI: 10.1038/s41598-021-04106-w
Abstrakt: Blood feeding and host-seeking behaviors of a mosquito play an imperative role in determining its vectorial capacity in transmitting pathogens. Unfortunately, limited information is available regarding blood feeding behavior of Anopheles species in Malaysia. Collection of resting Anopheles mosquitoes for blood meal analysis poses a great challenge especially for forest dwelling mosquitoes. Therefore, a laboratory-based study was conducted to evaluate the potential use of mosquitoes caught using human landing catch (HLC) for blood meal analysis, and subsequently to document blood feeding behavior of local Anopheles mosquitoes in Peninsular Malaysia. The laboratory-based experiment from this study revealed that mosquitoes caught using HLC had the potential to be used for blood meal analysis. Besides HLC, mosquitoes were also collected using manual aspirator and Mosquito Magnet. Overall, 47.4% of 321 field-caught Anopheles mosquitoes belonging to six species were positive for vertebrate host DNA in their blood meal. The most frequent blood meal source was human (45.9%) followed by wild boar (27.4%), dog (15.3%) and monkey (7.5%). Interestingly, only Anopheles cracens and Anopheles introlatus (Leucosphyrus Group) fed on monkey. This study further confirmed that members of the Leucosphyrus Group are the predominant vectors for knowlesi malaria transmission in Peninsular Malaysia mainly due to their simio-anthropophagic feeding behavior.
(© 2022. The Author(s).)
Databáze: MEDLINE
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