| Autor: |
Céspedes-Valenzuela DN; Grupo de Investigación en Nanobiomateriales, Ingeniería Celular y Bioimpresión (GINIB), Department of Biomedical Engineering, Universidad de los Andes, Bogota 111711, Colombia., Sánchez-Rentería S; Grupo de Investigación en Nanobiomateriales, Ingeniería Celular y Bioimpresión (GINIB), Department of Biomedical Engineering, Universidad de los Andes, Bogota 111711, Colombia., Cifuentes J; Grupo de Investigación en Nanobiomateriales, Ingeniería Celular y Bioimpresión (GINIB), Department of Biomedical Engineering, Universidad de los Andes, Bogota 111711, Colombia., Gantiva-Diaz M; Grupo de Investigación en Nanobiomateriales, Ingeniería Celular y Bioimpresión (GINIB), Department of Biomedical Engineering, Universidad de los Andes, Bogota 111711, Colombia.; Grupo de Investigación en Biomecánica (IBIOMECH), Department of Biomedical Engineering, Universidad de los Andes, Bogota 111711, Colombia., Serna JA; Grupo de Investigación en Nanobiomateriales, Ingeniería Celular y Bioimpresión (GINIB), Department of Biomedical Engineering, Universidad de los Andes, Bogota 111711, Colombia., Reyes LH; Department of Chemical and Food Engineering, School of Engineering, Universidad de Los Andes, Carrera 1 No. 18A-12, Bogota 111711, Colombia., Ostos C; Grupo CATALAD, Instituto de Química, Universidad de Antioquia, Medellin 050010, Colombia., Cifuentes-De la Portilla C; Grupo de Investigación en Biomecánica (IBIOMECH), Department of Biomedical Engineering, Universidad de los Andes, Bogota 111711, Colombia., Muñoz-Camargo C; Grupo de Investigación en Nanobiomateriales, Ingeniería Celular y Bioimpresión (GINIB), Department of Biomedical Engineering, Universidad de los Andes, Bogota 111711, Colombia., Cruz JC; Grupo de Investigación en Nanobiomateriales, Ingeniería Celular y Bioimpresión (GINIB), Department of Biomedical Engineering, Universidad de los Andes, Bogota 111711, Colombia. |
| Abstrakt: |
As life expectancy continues to increase, the inevitable weakening and rupture of bone tissue have grown as concerns in the medical community, thus leading to the need for adhesive materials suitable for bone repair applications. However, current commercially available adhesives face certain drawbacks that prevent proper tissue repair, such as low biocompatibility, poor adhesion to wet surfaces, and the need for high polymerization temperatures. This work aims to develop an injectable and photo-responsive chitosan methacrylate/graphene oxide (ChiMA/GO) adhesive nanocomposite hydrogel of high biocompatibility that is easy to apply by simple extrusion and that offers the possibility for in situ polymer and physiological temperatures. The nanocomposite was thoroughly characterized spectroscopically, microscopically, rheologically, thermally, and through mechanical, textural, and biological assays to fully evaluate its correct synthesis and functionalization and its performance under physiological conditions that mimic those observed in vivo. In addition, a finite element analysis (FEA) simulation was used to evaluate its performance in femur fractures. Results suggest the material's potential as a bioadhesive, as it can polymerize at room temperature, shows superior stability in physiological media, and is capable of withstanding loads from body weight and movement. Moreover, the material showed remarkable biocompatibility as evidenced by low hemolytic and intermediate platelet aggregation tendencies, and high cytocompatibility when in contact with osteoblasts. The comprehensive studies presented here strongly suggest that the developed hydrogels are promising alternatives to conventional bone adhesives that might be further tested in vivo in the near future. |
