| Autor: |
Kangwan N; Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand., Kongkarnka S; Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand., Boonkerd N; Division of Microbiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand., Unban K; Division of Biotechnology, School of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand., Shetty K; Global Institute of Food Security and International Agriculture (GIFSIA), Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA., Khanongnuch C; Division of Biotechnology, School of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand.; Research Center of Multidisciplinary Approaches to Miang, Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. |
| Abstrakt: |
This study aimed to investigate the protective effect of probiotics and synbiotics from traditional Thai fermented tea leaves (Miang) on dextran sulfate sodium (DSS)-induced colitis in mice, in comparison to sulfasalazine. C57BL/6 mice were treated with probiotics L. pentosus A14-6, CMY46 and synbiotics, L. pentosus A14-6 combined with XOS, and L. pentosus CMY46 combined with GOS for 21 days. Colitis was induced with 2% DSS administration for seven days during the last seven days of the experimental period. The positive group was treated with sulfasalazine. At the end of the experiment, clinical symptoms, pathohistological changes, intestinal barrier integrity, and inflammatory markers were analyzed. The probiotics and synbiotics from Miang ameliorated DSS-induced colitis by protecting body weight loss, decreasing disease activity index, restoring the colon length, and reducing pathohistological damages. Furthermore, treatment with probiotics and synbiotics improved intestinal barrier integrity, accompanied by lowing colonic and systemic inflammation. In addition, synbiotics CMY46 combined with GOS remarkedly elevated the expression of IL-10. These results suggested that synbiotics isolated from Miang had more effectiveness than sulfasalazine. Thereby, they could represent a novel potential natural agent against colonic inflammation. |
