Molecular Insights on Successful Reconstitution of Freeze-Dried Nanofibrillated Cellulose Hydrogel.

Autor: Koivunotko E; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland., Merivaara A; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland., Niemelä A; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland., Valkonen S; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland., Manninen K; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland., Mäkinen H; Department of Physics, University of Helsinki, P.O. Box 64, 00014 Helsinki, Finland., Viljanen M; Department of Physics, University of Helsinki, P.O. Box 64, 00014 Helsinki, Finland., Svedström K; Department of Physics, University of Helsinki, P.O. Box 64, 00014 Helsinki, Finland., Diaz A; Paul Scherrer Institute, Forschungsstrasse 111, 5232 Villigen, PSI, Switzerland., Holler M; Paul Scherrer Institute, Forschungsstrasse 111, 5232 Villigen, PSI, Switzerland., Zini J; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland., Paasonen L; UPM Biomedicals, UPM-Kymmene Corporation, 00100 Helsinki, Finland., Korhonen O; School of Phamacy, University of Eastern Finland, 70210 Kuopio, Finland., Huotari S; Department of Physics, University of Helsinki, P.O. Box 64, 00014 Helsinki, Finland., Koivuniemi A; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland., Yliperttula M; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland.
Jazyk: angličtina
Zdroj: ACS applied bio materials [ACS Appl Bio Mater] 2021 Sep 20; Vol. 4 (9), pp. 7157-7167. Date of Electronic Publication: 2021 Sep 01.
DOI: 10.1021/acsabm.1c00739
Abstrakt: The diversity and safety of nanofibrillated cellulose (NFC) hydrogels have gained a vast amount of interest at the pharmaceutical site in recent years. Moreover, this biomaterial has a high potential to be utilized as a protective matrix during the freeze-drying of heat-sensitive pharmaceuticals and biologics to increase their properties for long-term storing at room temperature and transportation. Since freeze-drying and subsequent reconstitution have not been optimized for this biomaterial, we must find a wider understanding of the process itself as well as the molecular level interactions between the NFC hydrogel and the most suitable lyoprotectants. Herein we optimized the reconstitution of the freeze-dried NFC hydrogel by considering critical quality attributes required to ensure the success of the process and gained insights of the obtained experimental data by simulating the effects of the used lyoprotectants on water and NFC. We discovered the correlation between the measured characteristics and molecular dynamics simulations and obtained successful freeze-drying and subsequent reconstitution of NFC hydrogel with the presence of 300 mM of sucrose. These findings demonstrated the possibility of using the simulations together with the experimental measurements to obtain a more comprehensive way to design a successful freeze-drying process, which could be utilized in future pharmaceutical applications.
Databáze: MEDLINE
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