| Autor: |
Liu S; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore., Wen F; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore.; Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325011, Zhejiang, People's Republic of China., Muthukumaran P; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore., Rakshit M; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore., Lau CS; Oral Health Academic Clinical Programme, Duke-NUS Medical School, Singapore 169857, Singapore.; Academic Clinical Programme Office (Research), National Dental Centre Singapore, Singapore 168938, Singapore., Yu N; Oral Health Academic Clinical Programme, Duke-NUS Medical School, Singapore 169857, Singapore.; Academic Clinical Programme Office (Research), National Dental Centre Singapore, Singapore 168938, Singapore., Suryani L; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore., Dong Y; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore., Teoh SH; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore.; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore. |
| Abstrakt: |
There is an urgent clinical need for wound dressings to treat skin injuries, particularly full-thickness wounds caused by acute and chronic wounds. Marine collagen has emerged as an attractive and safer alternative due to its biocompatibility, diversity, and sustainability. It has minimum risk of zoonotic diseases and less religious constraints as compared to mammalian collagen. In this study, we reported the development of a self-assembled nanofibrous barramundi ( Lates calcarifer ) collagen matrix (Nano-BCM), which showed good biocompatibility for full-thickness wound-healing applications. The collagen was extracted and purified from barramundi scales and skin. Thereafter, the physicochemical properties of collagen were systematically evaluated. The process to extract barramundi skin collagen (BC) gave an excellent 45% yield and superior purity (∼100%). More importantly, BC demonstrated structural integrity, native triple helix structure, and good thermal stability. BC demonstrated its efficacy in promoting human primary dermal fibroblast (HDF) and immortalized human keratinocytes (HaCaT) proliferation and migration. Nano-BCM has been prepared via self-assembly of collagen molecules in physiological conditions, which resembled the native extracellular matrix (ECM). The clinical therapeutic efficacy of the Nano-BCM was further evaluated in a full-thickness splinted skin wound mice model. In comparison to a clinically used wound dressing (DuoDerm), the Nano-BCM demonstrated significantly accelerated wound closure and re-epithelization. Moreover, Nano-BCM nanofibrous architecture and its ability to facilitate early inflammatory response significantly promoted angiogenesis and differentiated myofibroblast, leading to enhanced wound healing. Consequently, Nano-BCM demonstrates great potential as an economical and effective nonmammalian substitute to achieve skin regeneration. |
