Immunomodulatory functions of human mesenchymal stromal cells are enhanced when cultured on HEP/COL multilayers supplemented with interferon-gamma.

Autor:	Haseli M; Ralph E. Martin Department of Chemical Engineering, University of Arkansas, 3202 Bell Engineering Center, Fayetteville, AR, 72701, USA., Castilla-Casadiego DA; Ralph E. Martin Department of Chemical Engineering, University of Arkansas, 3202 Bell Engineering Center, Fayetteville, AR, 72701, USA.; Mcketta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA., Pinzon-Herrera L; Ralph E. Martin Department of Chemical Engineering, University of Arkansas, 3202 Bell Engineering Center, Fayetteville, AR, 72701, USA., Hillsley A; Mcketta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA., Miranda-Munoz KA; Department of Biomedical Engineering, College of Engineering, University of Arkansas, Fayetteville, AR, 72701, USA., Sivaraman S; Department of Biomedical Engineering, College of Engineering, University of Arkansas, Fayetteville, AR, 72701, USA., Rosales AM; Mcketta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA., Rao RR; Department of Biomedical Engineering, College of Engineering, University of Arkansas, Fayetteville, AR, 72701, USA., Almodovar J; Ralph E. Martin Department of Chemical Engineering, University of Arkansas, 3202 Bell Engineering Center, Fayetteville, AR, 72701, USA.
Jazyk:	angličtina
Zdroj:	Materials today. Bio [Mater Today Bio] 2021 Dec 23; Vol. 13, pp. 100194. Date of Electronic Publication: 2021 Dec 23 (Print Publication: 2022).
DOI:	10.1016/j.mtbio.2021.100194
Abstrakt:	Human mesenchymal stromal cells (hMSCs) are multipotent cells that have been proposed for cell therapies due to their immunosuppressive capacity that can be enhanced in the presence of interferon-gamma (IFN-γ). In this study, multilayers of heparin (HEP) and collagen (COL) (HEP/COL) were used as a bioactive surface to enhance the immunomodulatory activity of hMSCs using soluble IFN-γ. Multilayers were formed, via layer-by-layer assembly, varying the final layer between COL and HEP and supplemented with IFN-γ in the culture medium. We evaluated the viability, adhesion, real-time growth, differentiation, and immunomodulatory activity of hMSCs on (HEP/COL) multilayers. HMSCs viability, adhesion, and growth were superior when cultured on (HEP/COL) multilayers compared to tissue culture plastic. We also confirmed that hMSCs osteogenic and adipogenic differentiation remained unaffected when cultured in (HEP/COL) multilayers in the presence of IFN-γ. We measured the immunomodulatory activity of hMSCs by measuring the level of indoleamine 2,3-dioxygenase (IDO) expression. IDO expression was higher on (HEP/COL) multilayers treated with IFN-γ. Lastly, we evaluated the suppression of peripheral blood mononuclear cell (PBMC) proliferation when co-cultured with hMSCs on (HEP/COL) multilayers with IFN-γ. hMSCs cultured in (HEP/COL) multilayers in the presence of soluble IFN-γ have a greater capacity to suppress PBMC proliferation. Altogether, (HEP/COL) multilayers with IFN-γ in culture medium provides a potent means of enhancing and sustaining immunomodulatory activity to control hMSCs immunomodulation. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Authors.)
Databáze:	MEDLINE
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