The use of 68 Ga-EDTA PET allows detecting progressive decline of renal function in rats.

Autor:	Fontana AO; Department of Nuclear Medicine and Molecular Imaging, Lausanne University Hospital and University of Lausanne CH-1011 Lausanne, Switzerland., Gonzalez Melo M; Paediatric Metabolic Unit, Pediatrics, Woman-Mother-Child Department, University of Lausanne and University Hospital of Lausanne Switzerland., Allenbach G; Department of Nuclear Medicine and Molecular Imaging, Lausanne University Hospital and University of Lausanne CH-1011 Lausanne, Switzerland., Georgantas C; Department of Nuclear Medicine and Molecular Imaging, Lausanne University Hospital and University of Lausanne CH-1011 Lausanne, Switzerland., Wang R; Department of Nuclear Medicine and Molecular Imaging, Lausanne University Hospital and University of Lausanne CH-1011 Lausanne, Switzerland., Braissant O; Service of Clinical Chemistry, Lausanne University Hospital and University of Lausanne CH-1011 Lausanne, Switzerland., Barbey F; Department of Immunology, Lausanne University Hospital and University of Lausanne CH-1011 Lausanne, Switzerland., Prior JO; Department of Nuclear Medicine and Molecular Imaging, Lausanne University Hospital and University of Lausanne CH-1011 Lausanne, Switzerland., Ballhausen D; Paediatric Metabolic Unit, Pediatrics, Woman-Mother-Child Department, University of Lausanne and University Hospital of Lausanne Switzerland., Viertl D; Department of Nuclear Medicine and Molecular Imaging, Lausanne University Hospital and University of Lausanne CH-1011 Lausanne, Switzerland.
Jazyk:	angličtina
Zdroj:	American journal of nuclear medicine and molecular imaging [Am J Nucl Med Mol Imaging] 2021 Dec 15; Vol. 11 (6), pp. 519-528. Date of Electronic Publication: 2021 Dec 15 (Print Publication: 2021).
Abstrakt:	Introduction: Evaluation of glomerular filtration rate is very important in both preclinical and clinical setting, especially in the context of chronic kidney disease. It is typically performed using 51 Cr-EDTA or by imaging with 123 I-Hippuran scintigraphy, which has a significantly lower resolution and sensitivity as compared to PET. 68 Ga-EDTA represents a valid alternative due to its quick availability using a 68 Ge/ 68 Ga generator, while PET/CT enables both imaging of renal function and accurate quantitation of clearance of activity from both plasma and urine. Therefore, we aimed at investigating the use of 68 Ga-EDTA as a preclinical tracer for determining renal function in a knock-in rat model known to present progressive decline of renal function. Methods: 68 Ga-EDTA was injected in 23 rats, either wild type (n=10) or knock-in (n=13). By applying a unidirectional, two-compartment model and Rutland-Patlak Plot linear regression analysis, split renal function was determined from the age of 6 weeks to 12 months. Results: Glomerular filtration ranged from 0.025±0.01 ml/min at 6 weeks to 0.049±0.05 ml/min at 6 months in wild type rats. Glomerular filtration was significantly lower in knock-in rats at 6 and 12 months (P<0.01). No significant difference was observed in renal volumes between knock-in and wild type animals, based on imaging-derived volume calculations. Conclusions: 68 Ga-EDTA turned out to be a very promising PET/CT tracer for the evaluation of split renal function. This method allowed detection of progressive renal impairment in a knock-in rat model. Additional validation in a human cohort is warranted to further assess clinical utility in both, healthy individuals and patients with renal impairment. Competing Interests: None. (AJNMMI Copyright © 2021.)
Databáze:	MEDLINE
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