Indoleamine 2,3-Dioxygenase-1 Expression is Changed During Bladder Cancer Cell Invasion.

Autor: Santos HJSP; Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Matheus LHG; Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Silva A; Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Dalmazzo SV; Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Santos AA; Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Santos LRAR; Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Souza DM; Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Reis ST; Laboratory of Medical Investigation (LIM55), Urology Department, University of São Paulo Medical School, Sao Paulo, Brazil., Nascimento IP; Laboratório Especial de Desenvolvimento de Vacinas, Instituto Butantan, São Paulo, Brazil., Dellê H; Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Jazyk: angličtina
Zdroj: International journal of tryptophan research : IJTR [Int J Tryptophan Res] 2022 Jan 04; Vol. 15, pp. 11786469211065612. Date of Electronic Publication: 2022 Jan 04 (Print Publication: 2022).
DOI: 10.1177/11786469211065612
Abstrakt: The severity of the bladder carcinoma (BC) is directly linked to cell invasion and metastasis. Indoleamine 2,3-dioxygenase-1 (IDO-1) is an INF-γ-induced immunomodulating enzyme that has been linked to the cancer cell invasiveness. Because IDO1 is variable among the tumors, we analyzed its expression in the BC invasion using BC mice models and cell culture. MB49 cells were orthotopically or ectopically inoculated in C57Bl6 mice to evaluate IDO1 by immunohistochemistry. For in vitro experiments, expression of IDO1 and INF-γ was evaluated in grade-1 (RT4) and in grade-3 (T24) BC cell lines. Invading and non-invading T24 cells were separated using the Matrigel/Transwell system, of which total RNA was extracted immediately or after 2 weeks of subculture. Finally, IDO1 was silenced in T24 cells to verify its role on cell invasiveness. In both animal models, IDO1 was differentially expressed between non-invading and invading cells. In cell culture, T24 cells expressed more IDO1 than RT4 cells, independently of the INF-γ expression. IDO1 was differentially expressed between non-invading and invading T24 cells, a difference that was lost by long-time subculture. IDO1 silencing resulted in diminished cell invasiveness. In conclusion, IDO1 expression is changed during bladder carcinoma invasion, playing an important role in this process.
Competing Interests: Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(© The Author(s) 2022.)
Databáze: MEDLINE
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