| Autor: |
Mageed AA; Division of Pediatric Hematology/Oncology, Emory University, Atlanta, GA 30322., Findley HW Jr, Franco C, Singhapakdi S, Alvarado C, Chan WC, Ragab AH |
| Jazyk: |
angličtina |
| Zdroj: |
Cancer [Cancer] 1987 Dec 15; Vol. 60 (12), pp. 2913-8. |
| DOI: |
10.1002/1097-0142(19871215)60:12<2913::aid-cncr2820601212>3.0.co;2-j |
| Abstrakt: |
The purpose of this study was to determine (1) the number and activity of natural killer (NK) cells in children with acute leukemia at different stages of their disease; and (2) the effect of interleukin-2 (IL-2) in enhancing NK activity of these patients' cells. The mean percentage of Leu 11+ NK cells in patients at diagnosis (5% of peripheral blood (PB) mononuclear cells) was significantly lower than for patients on maintenance (23%), post-treatment (21%) and for normal children (20%). The mean PB NK cell cytotoxicity for patients at diagnosis (16% lysis versus K562) and during maintenance (20%) was significantly lower than for post-treatment (41%) and normal controls (40%). After NK cells were incubated for 5 days with IL-2, NK cells from 82% (36/44) of patients showed enhanced cytotoxicity toward K562 and several acute leukemia cell lines as well as toward autologous leukemic cells. Cytotoxicity toward autologous cells was very low (0% to 5%, 16 hour assay) before IL-2 stimulation, and significantly increased (23% to 69%) after stimulation, suggesting that IL-2 may be a useful agent for enhancing the antileukemic immune response. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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