Metagenomic and proteomic approaches in elucidating aflatoxin B 1 detoxification mechanisms of probiotic Lactobacillus casei Shirota towards intestine.

Autor: Liew WP; Department of Nutrition, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia., Sabran MR; Department of Nutrition, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia. Electronic address: mohdredzwan@upm.edu.my., Than LT; Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia., Abd-Ghani F; Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2022 Feb; Vol. 160, pp. 112808. Date of Electronic Publication: 2022 Jan 05.
DOI: 10.1016/j.fct.2022.112808
Abstrakt: The modulation of gut microbiota and proteome due to aflatoxin B 1 (AFB 1 ) by probiotics remains unclear. This study investigated the alterations of gut microbiota and proteome in AFB 1 -exposed rats treated with probiotic Lactobacillus casei Shirota (Lcs). Forty male Sprague Dawley rats were randomly divided into five groups (n = 8) comprised control, AFB 1 , AFB 1 +activated charcoal, AFB 1 +Lcs, and Lcs groups. The rats were subjected to different treatments via oral gavage for four weeks. Urine and serum were collected for the measurement of AFB 1 biomarkers and organs were harvested for histological analysis. Metagenomic sequencing was performed on fecal samples to profile gut microbiota. Besides, AFB 1 most affected organ i.e. jejunum was subjected to proteomic analysis. The results indicated that Lcs intervention significantly reduced AFB 1 biomarkers. H&E-stained intestine showed Lcs alleviated AFB 1 -induced inflammation and abnormal cell growth, particularly at the jejunum. Although AFB 1 increased potentially pathogenic bacteria and reduced beneficial bacteria abundance in feces, the microbiota composition was normalized with Lcs treatment. The gut proteome analysis of the jejunum sample showed several pathways of AFB 1 toxicity, wherein Lcs treatment demonstrated its protective effect. It is concluded that metagenomic and proteomic approaches are useful tools to understand AFB 1 -Lcs interaction and detoxification mechanism in the gut.
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Databáze: MEDLINE
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