| Autor: |
Thangavelu RM; Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India., Sundarajan D; Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India., Savaas Umar MR; Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India., Denison MIJ; Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India., Gunasekaran D; Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India., Rajendran G; Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India., Duraisamy N; Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India., Kathiravan K; Plant Molecular Virology and Nanobiotechnology Research Laboratory, Department of Biotechnology, University of Madras, Chennai, Tamil Nadu 600025, India. |
| Abstrakt: |
A new era has begun in which pathogens have become useful scaffolds for nanotechnology applications. In this research/study, an attempt has been made to generate an empty cargo-like architecture from a plant pathogenic virus named Squash leaf curl China virus (SLCCNV). In this approach, SLCCNV coat protein monomers are obtained efficiently by using a yeast Pichia pastoris expression system. Further, dialysis of purified SLCCNV-CP monomers against various pH modified (5-10) disassembly and assembly buffers produced a self-assembled "Nanocargo"-like architecture, which also exhibited an ability to encapsulate magnetic nanoparticles in vitro . Bioinformatics tools were also utilized to predict the possible self-assembly kinetics and bioconjugation sites of coat protein monomers. Significantly, an in vitro biocompatibility study using SLCCNV-Nanocargo particles showed low toxicity to the cells, which eventually proved as a potential nanobiomaterial for biomedical applications. |
