SARS-CoV-2 nucleocapsid protein adheres to replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress.

Autor: Scherer KM; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Mascheroni L; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Carnell GW; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK., Wunderlich LCS; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Makarchuk S; UK Dementia Research Institute, Cambridge, UK., Brockhoff M; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Mela I; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Fernandez-Villegas A; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Barysevich M; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Stewart H; Division of Virology, Department of Pathology, University of Cambridge, Cambridge, UK., Suau Sans M; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK., George CL; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK., Lamb JR; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Kaminski-Schierle GS; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK., Heeney JL; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK., Kaminski CF; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2022 Jan 07; Vol. 8 (1), pp. eabl4895. Date of Electronic Publication: 2022 Jan 07.
DOI: 10.1126/sciadv.abl4895
Abstrakt: Despite being the target of extensive research efforts due to the COVID-19 (coronavirus disease 2019) pandemic, relatively little is known about the dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication within cells. We investigate and characterize the tightly orchestrated virus assembly by visualizing the spatiotemporal dynamics of the four structural SARS-CoV-2 proteins at high resolution. The nucleoprotein is expressed first and accumulates around folded endoplasmic reticulum (ER) membranes in convoluted layers that contain viral RNA replication foci. We find that, of the three transmembrane proteins, the membrane protein appears at the Golgi apparatus/ER-to-Golgi intermediate compartment before the spike and envelope proteins. Relocation of a lysosome marker toward the assembly compartment and its detection in transport vesicles of viral proteins confirm an important role of lysosomes in SARS-CoV-2 egress. These data provide insights into the spatiotemporal regulation of SARS-CoV-2 assembly and refine the current understanding of SARS-CoV-2 replication.
Databáze: MEDLINE