Rational Design and Identification of Harmine-Inspired, N-Heterocyclic DYRK1A Inhibitors Employing a Functional Genomic In Vivo Drosophila Model System.

Autor:	Huizar FJ; Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA., Hill HM; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Bacher EP; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Eckert KE; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Gulotty EM; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Rodriguez KX; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Tucker ZD; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Banerjee M; Warren Family Center for Drug Discovery and Development, University of Notre Dame, Notre Dame, IN 46556, USA., Liu H; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA., Wiest O; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.; Warren Family Center for Drug Discovery and Development, University of Notre Dame, Notre Dame, IN 46556, USA., Zartman J; Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA., Ashfeld BL; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.; Warren Family Center for Drug Discovery and Development, University of Notre Dame, Notre Dame, IN 46556, USA.
Jazyk:	angličtina
Zdroj:	ChemMedChem [ChemMedChem] 2022 Feb 16; Vol. 17 (4), pp. e202100512. Date of Electronic Publication: 2022 Jan 27.
DOI:	10.1002/cmdc.202100512
Abstrakt:	Deregulation of dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) plays a significant role in developmental brain defects, early-onset neurodegeneration, neuronal cell loss, dementia, and several types of cancer. Herein, we report the discovery of three new classes of N-heterocyclic DYRK1A inhibitors based on the potent, yet toxic kinase inhibitors, harmine and harmol. An initial in vitro evaluation of the small molecule library assembled revealed that the core heterocyclic motifs benzofuranones, oxindoles, and pyrrolones, showed statistically significant DYRK1A inhibition. Further, the utilization of a low cost, high-throughput functional genomic in vivo model system to identify small molecule inhibitors that normalize DYRK1A overexpression phenotypes is described. This in vivo assay substantiated the in vitro results, and the resulting correspondence validates generated classes as architectural motifs that serve as potential DYRK1A inhibitors. Further expansion and analysis of these core compound structures will allow discovery of safe, more effective chemical inhibitors of DYRK1A to ameliorate phenotypes caused by DYRK1A overexpression. (© 2022 Wiley-VCH GmbH.)
Databáze:	MEDLINE
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