High-throughput, Label-free Proteomics Identifies Salient Proteins and Genes in MDA-MB-231 Cells Treated with Natural Neem-based Electrochemotherapy.

Autor: Varadarajan G; Department of Electrical Engineering, Divison of High Voltage Engineering, Anna University, Guindy, India., Thulasidas JS; Department of Electrical Engineering, Divison of High Voltage Engineering, Anna University, Guindy, India., Giri P; School of Engineering Technology, Purdue University, West Lafayette, IN, 47907, USA., Camarillo IG; Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA.; Purdue Center for Cancer Research, Purdue University, West Lafayette, IN, 47907, USA., Sundararajan R; School of Engineering Technology, Purdue University, West Lafayette, IN, 47907, USA. raji@purdue.edu.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2022 Jan; Vol. 194 (1), pp. 148-166. Date of Electronic Publication: 2022 Jan 06.
DOI: 10.1007/s12010-021-03787-3
Abstrakt: With the absence of the three most common receptor targets, and with high vascularity and higher-grade tumors, triple-negative breast cancer (TNBC) is the most aggressive of all breast cancer subtypes and is in need of additional/alternative/novel treatment strategies. With ~ 15% of the over 2 million new cases each year, there is an unmet need to treat TNBC. MDA-MB-231, human TNBC cells, were treated with neem leaf extract (Neem) and eight, 1200 V/cm, 100 µs electric pulses (EP), and their viability and proteomic profiles were studied. With EP + Neem, a lower viability of 37% was observed after 24 h, compared to 85% in the neem-only samples, indicating the efficacy of the combinational treatment. The proteomics results indicated significant upregulation of 525 proteins and downregulation of 572 proteins, with a number of different pathways in each case. These include a diverse group of proteins, such as receptors, heat shock proteins, and many others. The upregulated TCA cycle and OXPHOS pathways and the downregulated DNA replication and ubiquitin-mediated proteolytic pathways were associated with effective cell death, demonstrating the potency of this treatment. Viability results reveal the efficacious anticancer effects of the EP + Neem combination, via growth inhibition, on TNBC cells. Proteomics studies could readily identify the effected protein pathways, and their corresponding genes, that are responsible for cell death. This represents a potential therapeutic strategy against TNBC when patients are refractory to standard treatments.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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