The Linkage Phase of the Polymorphism KCNH2-K897T Influences the Electrophysiological Phenotype in hiPSC Models of LQT2.

Autor:	van den Brink L; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, Netherlands., Brandão KO; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, Netherlands., Yiangou L; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, Netherlands., Blanch-Asensio A; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, Netherlands., Mol MPH; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, Netherlands., Mummery CL; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, Netherlands.; Department of Applied Stem Cell Technologies, University of Twente, Enschede, Netherlands., Verkerk AO; Department of Medical Biology, Amsterdam UMC, Amsterdam, Netherlands., Davis RP; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, Netherlands.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2021 Dec 16; Vol. 12, pp. 755642. Date of Electronic Publication: 2021 Dec 16 (Print Publication: 2021).
DOI:	10.3389/fphys.2021.755642
Abstrakt:	While rare mutations in ion channel genes are primarily responsible for inherited cardiac arrhythmias, common genetic variants are also an important contributor to the clinical heterogeneity observed among mutation carriers. The common single nucleotide polymorphism (SNP) KCNH2-K897T is associated with QT interval duration, but its influence on the disease phenotype in patients with long QT syndrome type 2 (LQT2) remains unclear. Human induced pluripotent stem cells (hiPSCs), coupled with advances in gene editing technologies, are proving an invaluable tool for modeling cardiac genetic diseases and identifying variants responsible for variability in disease expressivity. In this study, we have used isogenic hiPSC-derived cardiomyocytes (hiPSC-CMs) to establish the functional consequences of having the KCNH2-K897T SNP in cis - or trans -orientation with LQT2-causing missense variants either within the pore-loop domain (KCNH2 A561T/WT ) or tail region (KCNH2 N996I/WT ) of the potassium ion channel, human ether-a-go-go-related gene (hERG). When KCNH2-K897T was on the same allele ( cis ) as the primary mutation, the hERG channel in hiPSC-CMs exhibited faster activation and deactivation kinetics compared to their trans -oriented counterparts. Consistent with this, hiPSC-CMs with KCNH2-K897T in cis orientation had longer action and field potential durations. Furthermore, there was an increased occurrence of arrhythmic events upon pharmacological blocking of hERG. Collectively, these results indicate that the common polymorphism KCNH2-K897T differs in its influence on LQT2-causing KCNH2 mutations depending on whether it is present in cis or trans . This study corroborates hiPSC-CMs as a powerful platform to investigate the modifying effects of common genetic variants on inherited cardiac arrhythmias and aids in unraveling their contribution to the variable expressivity of these diseases. Competing Interests: CM is a cofounder of Pluriomics B. V. (now Ncardia B. V.). The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 van den Brink, Brandão, Yiangou, Blanch-Asensio, Mol, Mummery, Verkerk and Davis.)
Databáze:	MEDLINE
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