SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish.

Autor: Brnić D; Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia. Electronic address: brnic@veinst.hr., Lojkić I; Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia., Škoko I; Croatian Veterinary Institute, Veterinary Department Split, Poljička cesta 33, 21000, Split, Croatia., Krešić N; Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia., Šimić I; Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia; Ruđer Bošković Institute, Bijenička cesta 54, 10000, Zagreb, Croatia., Keros T; Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia., Ganjto M; Zagreb Wastewater Ltd., Čulinečka cesta 287, 10040, Zagreb, Croatia., Štefanac D; Vodovod i kanalizacija d.o.o., Gažanski trg 8, 47000, Karlovac, Croatia., Viduka B; Odvodnja d.o.o., Hrvatskog sabora 2D, 23000, Zadar, Croatia., Karšaj D; Vodovod d.o.o., Nikole Zrinskog 25, 35000, Slavonski brod, Croatia., Štiler D; Vinkovački vodovod i kanalizacija d.o.o., Ulica Dragutina Žanića Karle 47a, 32100, Vinkovci, Croatia., Habrun B; Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia., Jemeršić L; Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia. Electronic address: jemersic@veinst.hr.
Jazyk: angličtina
Zdroj: Environmental research [Environ Res] 2022 May 01; Vol. 207, pp. 112638. Date of Electronic Publication: 2022 Jan 03.
DOI: 10.1016/j.envres.2021.112638
Abstrakt: The circulation of SARS-CoV-2 in the environment has been confirmed numerous times, whilst research on the bioaccumulation in bivalve molluscan shellfish (BMS) has been rather scarce. The present study aimed to fulfil the knowledge gap on SARS-CoV-2 circulation in wastewaters and surface waters in this region and to extend the current knowledge on potential presence of SARS-CoV-2 contamination in BMS. The study included 13 archive wastewater and surface water samples from the start of epidemic and 17 influents and effluents from nine wastewater treatment plants (WWTP) of different capacity and treatment stage, sampled during the second epidemic wave. From that period are the most of 77 collected BMS samples, represented by mussels, oysters and warty venus clams harvested along the Dalmatian coast. All samples were processed according to EN ISO 15216-1 2017 using Mengovirus as a whole process control. SARS-CoV-2 detection was performed by real-time and conventional RT-PCR assays targeting E, N and nsp14 protein genes complemented with nsp14 partial sequencing. Rotavirus A (RVA) real-time RT-PCR assay was implemented as an additional evaluation criterion of virus concentration techniques. The results revealed the circulation of SARS-CoV-2 in nine influents and two secondary treatment effluents from eight WWTPs, while all samples from the start of epidemic (wastewaters, surface waters) were negative which was influenced by sampling strategy. All tertiary effluents and BMS were SARS-CoV-2 negative. The results of RVA amplification were beneficial in evaluating virus concentration techniques and provided insights into RVA dynamics within the environment and community. In conclusion, the results of the present study confirm SARS-CoV-2 circulation in Croatian wastewaters during the second epidemic wave while extending the knowledge on wastewater treatment potential in SARS-CoV-2 removal. Our findings represent a significant contribution to the current state of knowledge that considers BMS of a very low food safety risk regarding SARS-CoV-2.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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