Organic β-cyclodextrin Nanoparticle: An Efficient Building Block Between Functionalized Poly(pyrrole) Electrodes and Enzymes.
Autor: | Buzzetti PHM; Univ. Grenoble Alpes, CNRS, DCM, Grenoble, 38000, France., Carrière M; Univ. Grenoble Alpes, CNRS, DCM, Grenoble, 38000, France.; Univ. Grenoble Alpes, CNRS, CERMAV, Grenoble, F-38000, France., Brachi M; Univ. Grenoble Alpes, CNRS, DCM, Grenoble, 38000, France., Gorgy K; Univ. Grenoble Alpes, CNRS, DCM, Grenoble, 38000, France., Mumtaz M; Univ. Grenoble Alpes, CNRS, CERMAV, Grenoble, F-38000, France., Borsali R; Univ. Grenoble Alpes, CNRS, CERMAV, Grenoble, F-38000, France., Cosnier S; Univ. Grenoble Alpes, CNRS, DCM, Grenoble, 38000, France. |
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Jazyk: | angličtina |
Zdroj: | Small (Weinheim an der Bergstrasse, Germany) [Small] 2022 Mar; Vol. 18 (10), pp. e2105880. Date of Electronic Publication: 2022 Jan 05. |
DOI: | 10.1002/smll.202105880 |
Abstrakt: | Glyconanoparticles (GNPs) made by self-assembly of carbohydrate-based polystyrene-block-β-cyclodextrin copolymer are used as a building block for the design of nanostructured biomaterials of electrode. The firm immobilization of GNPs is carried out on electrochemically generated polymer, poly(pyrrole-adamantane), and copolymer, poly(pyrrole-adamantane)/poly(pyrrole-lactobionamide) via host-guest interactions between adamantane and β-cyclodextrin. The ability of GNPs for the specific anchoring of biological macromolecules is investigated using glucose oxidase enzyme modified by adamantane groups as a protein model (GOx-Ad). The immobilization of GOx-Ad is carried out by incubation of an aqueous enzyme solution on a coating of GNPs adsorbed on a platinum electrode. The presence of immobilized GOx-Ad is evaluated in aqueous glucose solution by potentiostating the underlying platinum electrode at 0.7 V/SCE for the electro-oxidation of H (© 2022 Wiley-VCH GmbH.) |
Databáze: | MEDLINE |
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