Genomic analysis of Elizabethkingia species from aquatic environments: Evidence for potential clinical transmission.

Autor: Hem S; iThree Institute, University of Technology Sydney, Ultimo, NSW 2007, Australia.; Australian Centre for Genomic Epidemiological Microbiology, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia., Jarocki VM; iThree Institute, University of Technology Sydney, Ultimo, NSW 2007, Australia.; Australian Centre for Genomic Epidemiological Microbiology, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia., Baker DJ; Quadram Institute Bioscience, Norwich, United Kingdom., Charles IG; Quadram Institute Bioscience, Norwich, United Kingdom.; Norwich Medical School, Norwich Research Park, Colney Lane, Norwich NR4 7TJ, United Kingdom., Drigo B; Future Industries Institute, University of South Australia, Adelaide, SA 5001, Australia., Aucote S; Future Industries Institute, University of South Australia, Adelaide, SA 5001, Australia., Donner E; Future Industries Institute, University of South Australia, Adelaide, SA 5001, Australia., Burnard D; University of Queensland Centre for Clinical Research, Royal Brisbane and Woman's Hospital, Building 71/918 Royal Brisbane and Women's Hospital Campus, Herston, QLD 4029, Australia., Bauer MJ; University of Queensland Centre for Clinical Research, Royal Brisbane and Woman's Hospital, Building 71/918 Royal Brisbane and Women's Hospital Campus, Herston, QLD 4029, Australia., Harris PNA; University of Queensland Centre for Clinical Research, Royal Brisbane and Woman's Hospital, Building 71/918 Royal Brisbane and Women's Hospital Campus, Herston, QLD 4029, Australia., Wyrsch ER; iThree Institute, University of Technology Sydney, Ultimo, NSW 2007, Australia.; Australian Centre for Genomic Epidemiological Microbiology, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia., Djordjevic SP; iThree Institute, University of Technology Sydney, Ultimo, NSW 2007, Australia.; Australian Centre for Genomic Epidemiological Microbiology, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia.
Jazyk: angličtina
Zdroj: Current research in microbial sciences [Curr Res Microb Sci] 2021 Nov 26; Vol. 3, pp. 100083. Date of Electronic Publication: 2021 Nov 26 (Print Publication: 2022).
DOI: 10.1016/j.crmicr.2021.100083
Abstrakt: Elizabethkingia species are ubiquitous in aquatic environments, colonize water systems in healthcare settings and are emerging opportunistic pathogens with reports surfacing in 25 countries across six continents . Elizabethkingia infections are challenging to treat, and case fatality rates are high. Chromosomal bla B , bla GOB and bla CME genes encoding carbapenemases and cephalosporinases are unique to Elizabethkingia spp . and reports of concomitant resistance to aminoglycosides, fluoroquinolones and sulfamethoxazole-trimethoprim are known. Here, we characterized whole-genome sequences of 94 Elizabethkingia isolates carrying multiple wide-spectrum metallo-β-lactamase ( bla B and bla GOB ) and extended-spectrum serine‑β-lactamase ( bla CME ) genes from Australian aquatic environments and performed comparative phylogenomic analyses against national clinical and international strains. qPCR was performed to quantify the levels of Elizabethkingia species in the source environments. Antibiotic MIC testing revealed significant resistance to carbapenems and cephalosporins but susceptibility to fluoroquinolones, tetracyclines and trimethoprim-sulfamethoxazole. Phylogenetics show that three environmental E. anophelis isolates are closely related to E. anophelis from Australian clinical isolates (∼36 SNPs), and a new species, E. umeracha sp. novel , was discovered. Genomic signatures provide insight into potentially shared origins and a capacity to transfer mobile genetic elements with both national and international isolates.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2021 The Author(s).)
Databáze: MEDLINE
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