Clinical Outcomes of Rescue Intracytoplasmic Sperm Injection at Different Timings Following In Vitro Fertilization.
| Autor: | Shiraiwa Y; Hanabusa Women's Clinic, Hyogo, Japan., Enatsu N; Hanabusa Women's Clinic, Hyogo, Japan., Yamagami K; Hanabusa Women's Clinic, Hyogo, Japan., Furuhashi K; Hanabusa Women's Clinic, Hyogo, Japan., Iwasaki T; Hanabusa Women's Clinic, Hyogo, Japan., Otsuki J; Hanabusa Women's Clinic, Hyogo, Japan.; Okayama University Assisted Reproduction Technology Center, Okayama, Japan., Shiotani M; Hanabusa Women's Clinic, Hyogo, Japan. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of reproduction & infertility [J Reprod Infertil] 2021 Oct-Dec; Vol. 22 (4), pp. 251-257. |
| DOI: | 10.18502/jri.v22i4.7650 |
| Abstrakt: | Background: Although rescue intracytoplasmic sperm injection (r-ICSI) is extensively used worldwide, the indication of r-ICSI and its optimal timing remains obscure. This study aimed to assess the outcomes of r-ICSI following in vitro fertilization in different timings when fertilization is confirmed. Methods: This study included 5,156 cycles (47,785 eggs). Fertilization was confirmed by polar body analysis after 4 and 6 hr of coincubation of the sperm and oocyte. Oocytes that underwent IVF were divided into two groups based on the time when a second polar body was detected in more than 30% of all oocytes (Four- hr group and six- hr group). If the second polar body was not detected or was present in less than 30% of all oocytes after six hr of coincubation, rescue-ICSI (r-ICSI) was performed for oocytes without a second polar body (r-ICSI group). Results: The fertilization rates of two pronuclear (2PN) oocytes in the three groups (Four- hr group, six- hr group, and r-ICSI group) were 70.7%, 51.3%, and 58.0%, respectively. The blastocyst formation rates were 62.8%, 53.4%, and 42.9%, respectively. Conclusion: Performing r-ICSI after six hr of coincubation can salvage cases with fertilization failure in IVF. The higher fertilization rate of r-ICSI indicates that all oocytes without signs of fertilization after six hr of coincubation should undergo r-ICSI. Competing Interests: Conflict of Interest The authors declare that they have no competing interests. (Copyright© 2021, Avicenna Research Institute.) |
| Databáze: | MEDLINE |
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